[The correlation between NPHS2 polymorphism and IgA nephropathy in northern Chinese patients].

Objective: To examine the polymorphism in NPHS2 gene of IgA nephropathy in northern Chinese patients and to investigate the possible association of the NPHS2 polymorphism with the development of IgA nephropathy, as well as its clinical and histologic manifestations.

Methods: The polymorphism of NPHS2 was analyzed by direct DNA sequencing in 32 northern Chinese patients with IgA nephropathy (16 with heavy proteinuria and 16 with isolated hematuria). According to preliminary results, a total of 537 IgA nephropathy patients were genotyped for the NPHS2 C357T polymorphism by PCR combined with restriction fragment length polymorphism (PCR-RFLP).

[The clinical and pathological subtypes of Castleman's disease and their relationship with complications: a large series analysis from a single center].

Objectives: To investigate the clinical and pathological subtypes of Castleman's disease (CD) and their relationship with complications.

Methods: The clinical complications of 53 patients with CD and the relationship of these complications with clinical and pathological subtypes were analyzed retrospectively.

Results: Among 53 CD patients, 32 (60.

[Association of NPHS1 gene polymorphism with IgA nephropathy].

Objective: To investigate the association of the polymorphism of NPHS1, coding gene of nephrin, with the degree of proteinuria, renal function, and prognosis of IgA nephropathy (IgAN) in patients in north China.

Methods: Peripheral blood samples were collected from 532 patients with IgAN confirmed by biopsy, 285 males and 230 females, aged (31+/-11). Genomic DNA was isolated from the peripheral blood leucocytes.

[Approaches to detect the gene mutations in autosomal recessive Alport's syndrome: analysis of a family].

Objective: To explore the gene diagnostic method for autosomal recessive Alport syndrome (AR-AS).

Methods: Genomic DNA was extracted from the peripheral leukocytes of the proband of an AR-AS family. All the exons of COL4A3 and COL4A4 introns were amplified by PCR, and then the PCR products were sequenced by direct sequencing.

[Angiolymphoid hyperplasia with eosinophilia involving the kidney: a report of three cases with literature review].

Objective: To report the clinical and pathological characteristics of renal involvement in angiolymphoid hyperplasia with eosinophilia (ALHE).

Methods: Three cases of ALHE with renal involvement were diagnosed in our hospital. Routine pathological examination and immunohistochemical study of CD(31), CD(34) and F(8) of lymph node and renal biopsy specimens were performed and the clinical and pathological features were analysed.

[Clinical analysis of 8 cases with cryoglobulinemic glomerulonephritis].

Objective: To study the clinical and pathological features of cryoglobulinemic glomerulonephritis.

Methods: The clinical and pathologic data from 8 cases with cryoglobulinemic glomerulonephritis, which were referred to Peking University First Hospital from 2002 to 2006, were reviewed.

Results: There were seven males and one female, with an average age of 48.

[Variable number of tandem repeats polymorphism of MUC20 is associated with the progression of IgA nephropathy].

Objective: The MUC20 gene is a novel up-regulated gene that was identified in renal tissues of patients with IgA nephropathy (IgAN) by restriction endonucleolytic analysis of differentially expressed sequences. The variable number of tandem repeats (VNTR) polymorphism of MUC20 was detected in different cell lines. In this study we determined the distribution of MUC20 VNTR polymorphism in the healthy population, and the association between the MUC20 VNTR polymorphism and the pathogenesis or progression of IgAN.

[Uteroglobin G38A polymorphism is associated with the progression of IgA nephropathy in Chinese patients].

Objective: Uteroglobin is a multifunctional protein. Both uteroglobin gene knockout and antisense transgenic mouse models developed the pathological and clinical features of IgA nephropathy. Uteroglobin G38A polymorphism has been reported to be associated with the progression of IgA nephropathy in some Caucasian and Asian populations, but there are no documents on Chinese population.