The complete mitochondrial genome of Hand-Mazz (Ulmaceae) and phylogenetic analysis.

Hand-Mazz is a second-class key protected wild plant in China. Here, the complete mitochondrial genome of Hand-Mazz was sequenced using Nanopore Sequel and Illumina NovaSeq platform. The mitochondrial genome was assembled into three circular-mapping molecules with the genome sizes of 154,640 bp, 192,388 bp and 146,907 bp, including 36 protein-coding genes, 19 tRNA genes, and 3 rRNA genes.

Complete mitochondrial genome of (Ulmaceae) and phylogenetic analysis.

(Hance) Planch is a potential valuable forest tree in arid sandy environments. Here, the complete mitochondrial genome of was assembled using a combination of the PacBio Sequel data and the Illumina Hiseq data. The mitochondrial genome is 460,941 bp in length, including 37 protein-coding genes, 19 tRNA genes, and three rRNA genes.

PicW2 from Picea wilsonii: preparation, purification, crystallization and X-ray diffraction analysis.

Low temperature is a major limiting factor for plant growth and development. Dehydrin proteins are generally induced in response to low-temperature stress. In previous research, a full-length dehydrin gene, PicW2, was isolated from Picea wilsonii and its expression was associated with hardiness to cold.

[Virus resistance in transgenic watermelon plants containing a WMV-2 coat protein gene].

Virus disease is a major cause that affects the quality and output of watermelon which is an important fruit in summer. So it is really urgent to develop disease resistance plants. But it takes a long time to breed such plants in conventional ways, and it is very difficult to get ideal result.

[Identification of molecular markers associated with rice root traits by correlation coefficient analysis].

After growing in nutrient solution for 10 days, 84 rice varieties were harvested and measured in maximum root length (MRL) and root dry weight (RDW) separately. Twenty-seven of them were selected as representative variety for correlated fragments screening with amplified fragment length polymorphism (AFLP) technique. After surveying with 15 pairs of AFLP primers, 7 fragments from 4 pairs of primers were identified having significant correlation with MRL or/and RDW based on correlation coefficient analysis.

[Genetic analysis and molecular tagging of a gene for non-palea in rice].

The mutants involved in the development of floral organ are good material for understanding the molecular genetic mechanisms of floral development. A rice mutant, that lacks palea in its florets, was derived from a spontaneous mutation in an indica line, SAR III-93-369. Genetic analyses in three F2 populations from the mutant crossed with three rice varieties, Sheng 47, N625 and CDR22, respectively, showed that the mutant trait is controlled by a single recessive gene.