Simultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production by Clostridium saccharobutylicum DSM 13864.

Simultaneous saccharification and fermentation (SSF) process was applied for biobutanol production by Clostridium saccharobutylicum DSM 13864 from corn stover (CS). The key influential factors in SSF process, including corn steep liquor concentration, dry biomass and enzyme loading, SSF temperature, inoculation size and pre-hydrolysis time were optimized. In 5-L bioreactor with SSF process, butanol titer and productivity of 12.

Enhancing cellulose accessibility of corn stover by deep eutectic solvent pretreatment for butanol fermentation.

In this study, an effective corn stover (CS) pretreatment method was developed for biobutanol fermentation. Deep eutectic solvents (DESs), consisted of quaternary ammonium salts and hydrogen donors, display similar properties to room temperature ionic liquid. Seven DESs with different hydrogen donors were facilely synthesized.

Biobutanol production from corn stover hydrolysate pretreated with recycled ionic liquid by Clostridium saccharobutylicum DSM 13864.

In this study, corn stover (CS) hydrolysates, pretreated by fresh and recycled ionic liquid (IL) [Bmim][Cl], were utilized in butanol fermentation by Clostridium saccharobutylicum DSM 13864. An efficient CS pretreatment procedure using [Bmim][Cl] was developed, giving a glucose concentration of 18.7 g L(-1) using ten times recycled [Bmim][Cl], representing about 77% of that produced with fresh IL (24.