Application of stereotactic biopsy for diagnosing intracranial lesions in patients with AIDS in China: Report of 7 cases.

Rationale: The aim of the study was to evaluate stereotactic biopsy for diagnosing intracranial lesions in patients with AIDS.

Patient Concerns: Seven AIDS patients with an intracranial lesion who underwent stereotactic biopsy were included in this retrospective study (4 males and 3 females, 15 to 49 years old). The patients' disease history ranged from 1 month to 1 year.

Construction and functional activity of a recombinant vector expressing rat glutamic acid decarboxylase 65.

Objective: Glutamic acid decarboxylase 2 (GAD65) is a gamma-aminobutyric acid (GABA) synthetase. This study aimed to construct a recombinant lentivirus-rGAD65 (rLV-rGAD65) vector containing the cDNA of rat GAD65 (rGAD65) and assess its functional activity in vitro and in vivo.

Methods: cDNA of rGAD65 was amplified by RT-PCR and subcloned into the LV vector, forming the rLV-GFP-rGAD65 plasmid.

[Molecular characterization of full-length genome of Japanese encephalitis virus isolated in Liaoning Province in 2008].

Objective: To sequence and analyze the whole genome of Japanese encephalitis virus (JEV) isolated from mosquitoes in Liaoning province in 2008.

Methods: Using RT-PCR to amplify fragments with genome sequencing primer. The full-length genome was obtained by sequencing and splicing.

[Isolation and identification of arboviruses from mosquito pools in some regions of Liaoning province, China].

Objective: To isolate and identify arboviruses from mosquito pools in some regions of Liaoning province.

Methods: Mosquitoes were collected from Shenyang, Yingkou, Panjin, Jinzhou and Dandong cities of Liaoning province in 2006. Viruses were isolated by inoculating the specimens onto C6/ 36 and BHK-21cells.

Bis(ethyl-enediamine-κN,N')(nitrato-κO,O')cobalt(III) hydroxide nitrate.

The Co ion in the title salt, [Co(NO(3))(H(2)NCH(2)CH(2)NH(2))(2)](OH)(NO(3)), has oxidation state + III and is coordinated by four N atoms from two ethyl-enediamine mol-ecules and two O atoms from a nitrate anion in a distorted octa-hedral geometry. The charge of the complex cation is balanced by a hydroxide anion and a nitrate anion. The cations and anions are connected by N-H⋯O and O-H⋯O hydrogen bonds, resulting in a three-dimensional supra-molecular framework.

[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007].

Objective: To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.

Methods: Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells.

Non-Redundant Roles for Interleukin-1α and Interleukin-1β in Regulating Human IgG2.

Background: Serum concentrations of immunoglobulin G2 (IgG2) are elevated in localized aggressive periodontitis (LAgP) patients, and secretory products of monocytes from LAgP patients enhance IgG2 responses of lymphocytes from healthy subjects. Furthermore, genes regulating production of interleukin (IL)-1 influence the risk for both aggressive periodontitis (AgP) and chronic periodontitis. These observations, and the fact that IgG2 dominates responses to carbohydrates from Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis, prompted the hypothesis that IL-1α, IL-1β, and IL-RA may help regulate human IgG2 responses.

Antibody of the IgG2 Subclass, Actinobacillus actinomycetemcomitans, and Early-Onset Periodontitis.

Susceptibility to early-onset periodontitis (EOP) appears to be attributable to a gene inherited in an autosomal dominant pattern. This explains why EOP clusters in families and why about half of the family members develop periodontal disease early in life. Manifestation of EOP is variable, with some patients having a localized form restricted to first molars and incisors (LJP) and others with a severe generalized form of periodontitis (SP).