LncRNA NEAT1 regulates MMP-16 by targeting miR-200a/b to aggravate inflammation in asthma.

Asthma is a common respiratory disease which is characterized by persistent airway inflammation. Abnormal expression of long non-coding RNAs (lncRNAs) is observed in asthma. However, whether lncRNA nuclear-enriched abundant transcript 1 (NEAT1) regulates asthmatic inflammation and its mechanism still needs to be further investigated.

Lasting antibody and T cell responses to SARS-CoV-2 in COVID-19 patients three months after infection.

The dynamics, duration, and nature of immunity produced during SARS-CoV-2 infection are still unclear. Here, we longitudinally measured virus-neutralising antibody, specific antibodies against the spike (S) protein, receptor-binding domain (RBD), and the nucleoprotein (N) of SARS-CoV-2, as well as T cell responses, in 25 SARS-CoV-2-infected patients up to 121 days post-symptom onset (PSO). All patients seroconvert for IgG against N, S, or RBD, as well as IgM against RBD, and produce neutralising antibodies (NAb) by 14 days PSO, with the peak levels attained by 15-30 days PSO.

MiR-200a and miR-200b restrain inflammation by targeting ORMDL3 to regulate the ERK/MMP-9 pathway in asthma.

Asthma is one of the most frequent and serious diseases worldwide. Inflammation has been reported to correlate with airway remodeling, which is critical for the progression of asthma. Better understanding of novel molecules modulating asthma and the underlying mechanism will benefit explorations of new treatments.

[Different effects of long-term and short-term repeated restraints on the hematopoietic stem cells in mice].

Humans with chronic psychological stress are prone to develop multiple disorders of body function including impairment of immune system. Chronic psychological stress has been reported to have negative effects on body immune system. However, the underlying mechanisms have not been clearly demonstrated.

Gabapentin regulates dopaminergic neuron firing and theta oscillation in the ventral tegmental area to reverse depression-like behavior in chronic neuropathic pain state.

Purpose: Ventral tegmental area (VTA) dopamine system plays an important role in depression and is also involved in pain experience. In this study, we investigated the VTA dopaminergic (DA) neuron firing and local field potential (LFP) in pain-related depression, and we try to explore the underlying relationship between pain and depression.

Materials And Methods: We used neuropathic pain model [spare nerve injury (SNI)] to induce pain-related depression.

Acute and chronic pain affects local field potential of the medial prefrontal cortex in different band neural oscillations.

The medial prefrontal cortex is involved in the process of sensory discrimination. In this study, we examined the local field potential activity response to the different stages of pain in the prelimbic cortex (PrL) which is a sub-region of the medial prefrontal cortex. Recent studies revealed extensive information about neural oscillations, but there is limited information on the local field potential profiles for acute or chronic pain, particularly in freely moving animals.

Is gestational hypertension protective against perinatal mortality in twin pregnancies?

Background: Pregnancy-induced or gestational hypertension is a common pregnancy complication. Paradoxically, gestational hypertension has been associated with a protective effect against perinatal mortality in twin pregnancies in analytic models (logistic regression) without accounting for survival time. Whether this effect is real remains uncertain.

[Hyperactivation of c-Jun NH(2)-terminal protein kinase contributes to the proliferation of B lymphoma cells].

This study was purposed to explore the effect of hyperactivation of c-Jun NH(2)-terminal protein kinase (JNK) on the proliferation of B lymphoma cells. The human B lymphoma cell lines Daudi and Raji were chosen as research objects. The expression of JNK protein was determined by Western blot.

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation.

Mesenchymal stem cells (MSCs) represent powerful tools for regenerative medicine for their differentiation and migration capacity. However, ontogeny and migration of MSCs in mammalian mid-gestation conceptus is poorly understood. We identified canonical MSCs in the mouse embryonic day (E) 11.

Interleukin-3 promotes hemangioblast development in mouse aorta-gonad-mesonephros region.

Background: The hemangioblast is a bi-potential precursor cell with the capacity to differentiate into hematopoietic and vascular cells. In mouse E7.0-7.

The characteristic of an anti-human DR5 antibody A6.

The efficacy of many cancer treatments is due to their ability to induce apoptosis. DR5 can activate apoptosis pathway after binding with its natural ligand, tumour necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L). Both TRAIL and agonistic anti-DR5 monoclonal antibody are currently being explored for cancer therapy.

Deactivation of the kinase IKK by CUEDC2 through recruitment of the phosphatase PP1.

Despite rapid progress in elucidating the molecular mechanisms of activation of the kinase IKK, the processes that regulate IKK deactivation are still unknown. Here we demonstrate that CUE domain-containing 2 (CUEDC2) interacted with IKKalpha and IKKbeta and repressed activation of the transcription factor NF-kappaB by decreasing phosphorylation and activation of IKK. Notably, CUEDC2 also interacted with GADD34, a regulatory subunit of protein phosphatase 1 (PP1).

Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells.

Cytokine-induced differentiation of myeloid leukemia cells has important therapeutic implications, but the mechanism remains to be clarified. M1 cell, a mouse acute myeloid leukemia cell line, which underwent growth inhibition, terminal differentiation and apoptosis in response to IL-6, was selected as an experimental model to study on the molecular mechanisms of myeloid cell differentiation on a proteome-wide scale. Cell differentiation was evaluated by cell morphology and CD11b expression.

Comparison of IL-6-induced STAT1 activation in different responsive cells.

Aim: To investigate the activation status of signal transducer and activator of transcription 1 (STAT1) induced by interlerkin-6 (IL-6) in different responsive cells.

Methods: Immunoblot analysis was performed with antibodies against STAT1 and tyrosine-phosphorylated STAT1.

Results: It's found that IL-6 had growth-promoting effects on 7TD1 and TF1 cells but had little effect on the tyrosine-phosphorylation of STAT1.

PI3-K/Akt pathway contributes to IL-6-dependent growth of 7TD1 cells.

BACKGROUND: Recently, growing evidence suggests the involvement of PI 3-K/Akt in IL-6-dependent survival and proliferative responses in several types of cells. However, whether PI 3-K/Akt plays the same role in IL-6-dependent growth of 7TD1 mouse-mouse B cell hybridoma is not known. METHODS: We investigated the activation status of Akt in 7TD1 cells induced by IL-6.

Phosphatidylinositol(PI)-3 Kinase Contributes to the Activation of Stat3 in M1 Leukemia Cells Induced by IL-6.

Wortmannin, a specific inhibitor of PI-3 kinase, antagonized the inhibitory effect of IL-6 in M1 acute myeloid leukemia cells. Electrophoretic mobility shift assays revealed that wortmannin selectively reduced the IL-6-induced activation of Stat3, while Stat3 plays a central role in IL-6-induced growth-arrest and terminal differentiation of M1 cells. These results indicated that PI-3 kinase was involved in the signal transduction of IL-6 and contributed to the activation of Stat3.

Calibration error on the measurement of back vertex power for contact lenses with method using focimeter with manual focusing.

Purpose: The International Standard ISO 9337-1, which sets forth the method for measuring back vertex power of contact lenses with manually focusing focimeters, specifies that test lenses conforming to ISO 9342 may be used to calibrate focimeters on its spectacle lens support, and correction values obtained in this way can be used directly in measuring contact lenses on its contact lens support. This study was conducted because of concern that the method mentioned in ISO 9337-1 is not good enough for the calibration of focimeters used to measure contact lenses.

Methods: To test the validity of this method, a research group from China National Institute of Metrology (NIM) studied it theoretically and carried out a series of comparison experiments, respectively, with the conventional test lenses conforming to ISO 9342 and test lenses made at NIM (with an expanded uncertainty of 0.