Vps4a Mediates a Unified Membrane Repair Machinery to Attenuate Ischemia/Reperfusion Injury.

Background: Cardiac ischemia/reperfusion disrupts plasma membrane integrity and induces various types of programmed cell death. The ESCRT (endosomal sorting complex required for transport) proteins, particularly AAA-ATPase Vps4a (vacuolar protein sorting 4a), play an essential role in the surveillance of membrane integrity. However, the role of ESCRT proteins in the context of cardiac injury remains unclear.

Comparative genomics of Tn6411 transposons carrying the blaIMP-1 gene in Pseudomonas aeruginosa.

We aimed to determine the molecular characteristics of carbapenem-resistant Pseudomonas aeruginosa strains 18081308 and 18083286, which were isolated from the urine and the sputum of two Chinese patients, respectively. Additionally, we conducted a comparative analysis between Tn6411 carrying blaIMP-1 in strain 18083286 and transposons from the same family available in GenBank. Bacterial genome sequencing was carried out on strains 18081308 and 18083286 to obtain their whole genome sequence.

Prevalence of Antibiotic Resistance and Virulence Genes in Carried by Migratory Birds on the Inner Mongolia Plateau of Northern China from 2018 to 2023.

(1) Background: Antibiotic resistance in bacteria is an urgent global threat to public health. Migratory birds can acquire antibiotic-resistant and pathogenic bacteria from the environment or through contact with each other and spread them over long distances. The objectives of this study were to explore the relationship between migratory birds and the transmission of drug-resistant pathogenic .

Liquid biopsy for human cancer: cancer screening, monitoring, and treatment.

Currently, tumor treatment modalities such as immunotherapy and targeted therapy have more stringent requirements for obtaining tumor growth information and require more accurate and easy-to-operate tumor information detection methods. Compared with traditional tissue biopsy, liquid biopsy is a novel, minimally invasive, real-time detection tool for detecting information directly or indirectly released by tumors in human body fluids, which is more suitable for the requirements of new tumor treatment modalities. Liquid biopsy has not been widely used in clinical practice, and there are fewer reviews of related clinical applications.

Metagenomic and Antibiotic Resistance Analysis of the Gut Microbiota in and Species Inhabiting the Honghaizi Wetland of Ordos, Inner Mongolia, from 2021 to 2023.

Gut microbes thrive by utilising host energy and, in return, provide valuable benefits, akin to a symbiotic relationship. Here, metagenomic sequencing was performed to characterise and compare the community composition, diversity and antibiotic resistance of the gut microbiota of Relict gull () and species. Alpha diversity analysis revealed that the intestinal microbial richness of was significantly lower than that of , with distinct differences observed in microbial composition.

Overexpression of Improved Cadmium Accumulation and Tolerance in Tobacco ( L.).

Cadmium (Cd)-induced oxidative stress detrimentally affects hyperaccumulator growth, thereby diminishing the efficacy of phytoremediation technology aimed at Cd pollution abatement. In the domain of plant antioxidant mechanisms, the role of glutathione peroxidase (GPX) in conferring Cd tolerance to tobacco () remained unclear. Our investigation employed genome-wide analysis to identify 14 genes in tobacco, revealing their organization into seven subgroups characterized by analogous conserved domain patterns.

A with a novel type of -carrying plasmid isolated from a zebra in China.

Background: is an emerging fly-borne zoonotic pathogen, which causes infections in immunocompromised patients and some animals. Herein, we reported a BM-Y from a dead zebra in China.

Methods: The complete genome sequencing of BM-Y showed that this isolate carried one chromosome and one novel type of -carrying plasmid.

Large deflection analysis of circular piezoelectric micro-actuator with flexoelectric effect.

At micro/nano scale, the stiffening effect and flexoelectric effect of strain gradient play important roles in the electromechanical coupling response of piezoelectric micro-components. In this paper, the large deflection bending problem of circular piezoelectric micro-actuator is studied based on the extended linear dielectric theory. In addition to the piezoelectric effect, the flexoelectric effect, the stiffening effect of strain gradient and the high-order electric field effect of polarization gradient are introduced.

Does saponin in quinoa really embody the source of its bitterness?

While it is widely reported that saponins are the main source of the bitter taste in quinoa, this work found that some saponin compounds in quinoa husks elicit an umami response. The saponins were analyzed qualitatively and quantified by mass spectrometry (UPLC-MS). Two quinoa saponin compounds RT 46 (3-O-β-d-glucopyranosyl-(1 → 3)-α-l-arabino-pyranosyl-phytolaccagenic acid 28-O-β-d-gluco-pyranosyl), and RT 53 (3-O-β-d-glucopyranosyl-(1 → 4)-β-d-glucopyranosyl-28-O-hederagenin) were isolated from quinoa husks through separation and purification.

Self-assembled carboxymethyl chitosan/zinc alginate composite film with excellent water resistant and antimicrobial properties for chilled meat preservation.

A major way to reduce meat waste is to extend the shelf life of chilled meat with appropriate packaging. However, most of the packaging film cannot keep meat fresh because of its poor antibacterial and water resistance performance. In this paper, a composite film for chilled meat packaging was synthesized by simple self-assembly of zinc ions with chelating carboxyl groups.

Comparative Transcriptome Analysis Reveals the Effect of miR156a Overexpression on Mineral Nutrient Homeostasis in .

Mineral nutrition plays an important role in crop growth, yield and quality. MiR156 is a regulatory hub for growth and development. To date, the understanding of miR156-mediated mineral homeostasis is limited.

Molecular characterization and antimicrobial susceptibility of human in Northeast China.

Introduction: Northeast China has always been an area with severe brucellosis prevalence. This study will identify in Northeast China and test its resistance to antibiotics, in order to clarify its resistance mechanism. is a widespread and highly pathogenic bacteria that poses serious threats to public health and animal husbandry.

Prevalence of antimicrobial resistance and virulence genes in Klebsiella pneumoniae and Congenetic Raoultella Isolates from captive giant pandas.

To study antimicrobial resistance and virulence genes of Klebsiella pneumoniae and Raoultella strains isolated from captive giant pandas. Non-duplicate fecal samples were collected from 128 giant pandas during 2017-2019. All isolated microbial strains were tested for antimicrobial drug susceptibility using BD verification panels.

Virulence genes and antimicrobial resistance in Enterococcus strains isolated from dogs and cats in Northeast China.

This study aimed to characterize the antimicrobial resistance and virulence of Enterococcus from dogs and cats in Northeast China and evaluate its zoonotic risk based on a total of 469 enterococci strains from 610 samples, including 238 strains of E. faecium and 128 strains of E. faecalis.

Virulence, Antibiotic Resistance, and Phylogenetic Relationships of spp. Carried by Migratory Birds in China.

This study aimed to evaluate antimicrobial resistance, virulence, and the genetic diversity of isolated from migratory birds from Guangxi Province, Guangdong Province, Ningxia Hui Autonomous Region, Jiangxi Province, and Inner Mongolia in China. A total of 810 samples were collected, including fresh feces, cloacal swabs, and throat swabs. The collected samples were processed and subjected to bacteriological examination.

Complete genome sequence of a bla-producing Escherichia coli DC71 assigned as ST410-O8:H9 and recovered from a captive giant panda (Ailuropoda melanoleuca) in China.

Objectives: In this study, we report the complete genome sequence of a multidrug-resistant Escherichia coli strain recovered from a fecal sample from a captive giant panda in China.

Methods: Antimicrobial susceptibility testing was performed. Genomic DNA from E.

Antimicrobial Resistance and Prevalence of Extended Spectrum β-Lactamase-Producing from Dogs and Cats in Northeastern China from 2012 to 2021.

(1) Background: there has been a growing concern about pet-spread bacterial zoonosis in recent years. This study aimed to investigate the trend in drug-resistance of canine isolates in northeast China between 2012-2021 and the differences in drug-resistance of of different origins in 2021. (2) Methods: were isolated from feces or anal swab samples from dogs and cats, and their antibiotic susceptibility profiles and phylogenetic grouping were identified.

A clinical strain with carried by an integrative and conjugative element in China.

Objective: To precisely determine the species of a carbapenem-resistant strain 1809276 isolated from the urine of a Chinese patient and analyze its integrative and conjugative element (ICE) formation mechanism.

Methods: Single-molecule real-time (SMRT) sequencing was carried out on strain 18091276 to obtain the complete chromosome and plasmid (pCN1276) sequences, and average nucleotide identity (ANI) was used for precise species identification. The ICEs in GenBank with the same integrase structure as ICE were aligned.

First Report of the Colistin Resistance Gene Carried by Inc Plasmid pSL12517-mcr10.1 in Enterobacter cloacae in Sierra Leone.

Mobile colistin resistance () gene has been distributed widely since it was initially identified in 2020. The aim of this study was to report the first in Africa and the first in Sierra Leone; furthermore, we presented diverse modular structures of loci. Here, the complete sequence of one -carrying plasmid in one clinical Enterobacter cloacae isolate from Sierra Leone was determined.

The Superoxide Dismutase Gene Family in : Genome-Wide Identification, Characterization, Expression Profiling and Functional Analysis in Response to Heavy Metal Stress.

Superoxide dismutases (SODs) play an important role in protecting plants against ROS toxicity induced by biotic and abiotic stress. Recent studies have shown that the SOD gene family is involved in plant growth and development; however, knowledge of the SOD gene family in tobacco is still limited. In the present study, the SOD gene family was systematically characterized in the tobacco genome.

Animal Models for Postoperative Implant-Related Spinal Infection.

Postoperative infections following implant-related spinal surgery are severe and disastrous complications for both orthopaedic surgeons and patients worldwide. They can cause neurological damage, disability, and death. To better understand the mechanism of these destructive complications and intervene in the process, further research is needed.