Effects of bacteria on early-stage litter decomposition in Wudalianchi volcanic forest.

To understand the role of microorganisms in litter decomposition and nutrient cycling in volcanic forest ecosystem, we conducted litterbag decomposition experiment and used Illumina MiSeq high-throughput sequencing to analyze the response of bacterial community structure and diversity during the decomposition of litters from , and , the dominant tree species in volcanic lava plateau of Wudalianchi. The results showed that mass remaining percentage of litters of three species after 18-month decomposition was 63.9%-68.

Competitive and sequence reactions of typical hydrocarbon molecules in diesel fraction hydrocracking - a theoretical study by DFT calculations.

The molecular structures of hydrocarbon molecules determine the competitive and sequence reactions in the diesel hydrocracking process. In this study, the hydrocracking reactions of typical hydrocarbons with various saturation degrees and molecular weights in diesel fractions synergistically catalyzed by the Ni-Mo-S nanocluster and Al-Si FAU zeolite are investigated. The results show that the two major rate-controlling steps in saturated hydrocarbon hydrocracking are dehydrogenation on the Ni-Mo-S active sites and the cracking of the C-C bonds on the FAU zeolite acid center.

[Effects of water levels in heterogeneous habitats on sexual reproductive allocation of ].

Taking as the research object, a representative plant of wetland in Sanjiang Plain, we analyzed the variations of individual size and biomass among the habitats of swamp wetlands, swampy meadows, typical meadows and miscellaneous grass meadows, and the relationship between reproductive components and plant biomass. We explored the effects of water level on individual biomass and reproductive allocation of in different types of wetlands. The results showed that plant biomass, height and the characteristics of sexual reproduction significantly decreased with the increases of water level.

Evaluation of dietary Perilla frutescens seed on performance and carcass quality in finishing castrated male Songliao black pigs.

Objectives: This study was conducted to investigate the effects of dietary supplementation of Perilla frutescens seed (PFS) on growth performance, blood profiles, meat quality and meat nutrient characteristics in finishing castrated male Songliao black pigs.

Methods: A total of 80 castrated male Songliao black pigs with an average initial body weight (BW) of 84.1 ± 2.

Antiapoptotic effects of velvet antler polypeptides on damaged neurons through the hypothalamic-pituitary-adrenal axis.

We investigated the effects of velvet antler polypeptide on cognitive impairment and the underlying mechanisms. Hydrogen peroxide-induced cell injury was used to establish an model of SH-SY5Y cells. In addition, we established an mouse model of cognitive impairment using intraperitoneal injections of scopolamine hydrobromide in strain mice.

[Fungal Community Structure of Different Degeneration Wetlands in Sanjiang Plain].

To understand the wetland soil fungal community structure and diversity in different degeneration wetlands, the topsoil (0-20) of three different degeneration wetlands were collected in the Sanjiang Plain field experiment station of the Institute of Nature and Ecology, Heilongjiang Academy of Sciences. The distribution and variation of soil fungal diversity were assessed by high-throughput sequencing method. The results showed that Shannon-Wiener index increased from marsh wetland marsh meadow wetland meadow wetland.

[Cnb1 involved in cytokinesis in Schizosaccharomyces pombe].

Serine/Threonine-specific calcineurin (CN) is highly conserved in eukaryotes, which plays an important role in transcriptional regulation. In Schizosaccharomyces pombe, CN exists as a heterodimer composed by catalytic subunit Ppb1 and regulatory subunit Cnb1. Deletion of cnb1+ reduced the growth rate of cells, and caused a chained phenotype, and had delay in cytokinesis.

[Experimental study on effect and mechanism of soybean protease inhibitor on endotoxin-induced acute lung injury].

Objective: To study the protective effect of soybean protease inhibitor on LPS-induced lung injury in rats.

Method: Fifty male SD rats were randomly divided in five groups, 10 rats in each group as sham-operation group, model control group, positive medicine group, and high, moderate SBTI groups. Except the sham-group, other groups were induced by intratracheal instillation of LPS with a dose of 6 mg x kg(-1).

[Effects of curcumin on bleomycin-induced damages in pulmonary functions in rats].

Objective: To determine the effects of curcumin on bleomycin (BLM)-induced pulmonary fibrosis in rats.

Method: One hundred and forty-four male Sprague-Dawley rats were randomized into 6 groups (24 rats in each group, model group, sham group, prednisone group (0.56 mg x kg(-1) x d(-1)), curcumin with low dose 5 mg group, curcumin with middle dose group 10 mg and curcumin with high dose group 20 mg per 100 g of body weight).

[Apoptosis of type II alveolar epithelial cell induced by bleomycin in lung fibrotic rat].

Objective: To investigate the effects of bleomycin (BLM) on the apoptosis of type II alveolar epithelial cell (AT II) in lung fibrotic rats and its possible mechanisms.

Methods: Totally 32 male Sprague-Dawley rats were randomly divided into sham group (n = 8) and BLM group (n = 24). Rats in sham group or BLM group were intratracheally instillated with saline or 5 mg/kg of bleomycin, respectively.

[Study on role of Chengqi Shengxue prescription in anti-tumor and immunoregulation].

Objective: To investigate the role of Chengqi Shengxue prescription in anti-tumor and immunoregulation and to evaluate its effect on apoptosis and T lymphocyte subsets of tumor-bearing mice.

Method: S180 ascites tumor and Lewis lung carcinoma tumor-bearing mice were used in the screening. Then 55 mice were treated randomly with the model, cyclophosphamide (30 mg x kg(-1)), or three different dosages of Chengqi Shengxue prescription (2.

[Research on phytoestrogenic effects and its mechanism of psoralen].

Objective: To observe and evaluate the phytoestrogenic effects and its mechanism of psoralen in estrogen receptor (ER) alpha and beta positive T47D and ishikawa cells.

Method: The proliferation rate of T47D influenced by 1 x 10(-5) mol x L(-1) to 1 x 10(-9) mol x L(-1) psoralen and that of Ishikawa influenced by 1 x 10(-6) mol x L(-1) and 1 x 10(-7) mol x L(-1) psoralen were analyzed by MTT assay. PR mRNA expression in T47D was quantified by RT-PCR assay.

[Treatment of Alzheimer disease: an evidence-based review].

Objective: The purpose of this research was mainly two-fold: first, to get an understanding of current researches conducted on Alzheimer disease in China; second, to systematically evaluate and compare Alzheimer's treatment delivered by traditional Chinese medicine (TCM) and Western medicine.

Methods: Two steps were employed in this research. They were data collection and cleaning, followed by systemic review and qualitative analysis.

Disruption of the intracellular Ca2+ homeostasis in the cardiac excitation-contraction coupling is a crucial mechanism of arrhythmic toxicity in aconitine-induced cardiomyocytes.

Aconitine is an effective ingredient in Aconite tuber, an important traditional Chinese medicine. Aconitine is also known to be a highly toxic diterpenoid alkaloid with arrhythmogenic effects. In the present study, we have characterized the properties of arrhythmic cytotoxicity and explored the possible mechanisms of aconitine-induced cardiomyocytes.

[Changes of matrix metalloproteinases activities in pulmonary fibrosis rats].

Objective: To observe the changes of matrix metalloproteinases (MMPs) activities in pulmonary fibrosis rats.

Methods: Eighty male SD rats were randomly divided into sham group (n = 40) and bleomycin group (BLM, n = 40), in which SD rats were injected with a single intratracheal dose of sham saline or bleomycin respectively. On day 1, 3, 7, 14, and 28 following bleomycin or saline instillation, rats were randomly killed, and serum from abdominal aorta, alveolar fluid from the bronchoalveolar lavage, and the lung homogenate were collected and then stored at -80 degrees C.

[Experimental study of estrogenic activities of five kinds of Chinese herbal medicines].

Objective: To evaluate the estrogenic activity of several kinds of Chinese herbal medicines.

Method: Use zoopery and reporter gene technique to study the estrogenic activity of five Chinese herbal medicines. Zoopery: weanling female Kunming mice weight 9-12 g were administrated botanical extracts of Selaginella tamariscina, Pinus Massoniana, Corallodiscus flabellate, Dryopteris sublaeta and Leonurus heterophyllus, the positive control group with Nilestriol tablets and control group with water, respectiely.

[Experimental study on effect of curcumin on inhibiting injury of free radical in pulmonary fibrosis rats].

Objective: To study on the effect and mechanism of curcumin on inhibiting injury induced by free radical in pulmonary fibrosis.

Method: One hundred and forty-four male SD rats were randomly divided into 6 groups (24 rats in each group). Rats in the model control group, positive medicine group, and high, moderate and low curcumin groups were injected with a single dose of bleomycin by trachea, and rats in sham-model control group with same volume normal saline.

[Experimental study on protective effects of curcumin on exaggerated extracellular matrix accumulation of pulmonary fibrosis rats].

Objective: To study the protective effects of curcumin on exaggerated extracellular matrix accumulation of pulmonary fibrosis rats.

Method: One hundred and forty-four male Sprague-Dawley rats were randomly divided into 6 groups (24 rats in each group). Rats in the model control group, positive medicine group, and high, moderate and low curcumin groups were injected with a single dose of bleomycin by trachea, and rats in sham-model control group with same volume normal saline.

Pathological mechanisms of alcohol-induced hepatic portal hypertension in early stage fibrosis rat model.

Aim: To study the role of hepatic sinusoidal capillarization and perisinusoidal fibrosis in rats with alcohol-induced portal hypertension and to discuss the pathological mechanisms of alcohol-induced hepatic portal hypertension.

Methods: Fifty SD rats were divided into control group (n=20) and model group (n=30). Alcoholic liver fibrosis rat model was induced by intragastric infusion of a mixture containing alcohol, corn oil and pyrazole (1 000:250:3).

[The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice].

Objective: To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.

Method: The animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray.

[Rat alveolar type II injured by bleomycin].

Objective: To explore dysfunction mechanism of rat alveolar type II (AT-II) injured by bleomycin (BLM).

Methods: SD rats were injected with a single intratracheal dose of bleomycin or control saline. On day 7, 14, and 28 following intratracheal bleomycin or saline instillation, animals were killed under overdose of 1.

[The protective effects of Fufang Biejiafang on bleomycin-induced pulmonary fibrosis in rats].

Objective: To explore the possible effects and mechanism of Fufang Biejiafang on a single intratracheal instillation (IT) of bleomycin-induced lung fibrosis model.

Method: SD rats were treated with a single IT dose of bleomycin or control saline. Chinese medicine group were poured into the stomach after the first day of operation with high dosage, middle dosage and low dosage.

[Influence of compound biejia ruangan prescription on extracelluar matrix in bleomycin induced pulmonary fibrosis rats].

Objective: To study the influence of compound Biejia Ruangan Prescription (CBRP) on extracelluar matrix in bleomycin induced pulmonary fibrosis rats.

Method: 54 male Sprague-Dawley rats were randomly divided into 6 groups (9 rats in each group). Rats in the model control group, positive medicine group, and high, moderate and low CBRP groups were injected with bleomycin A5 by trachea, and rats in sham-model control group with same volume normal saline.

[The isolation and identification of a new lignanoside from Selaginella tamariscina (Beauv.) Spring].

Aim: To study the chemical constituents of Selaginella tamariscina (Beauv.) Spring.

Methods: The compounds were isolated and purified by macroporous adsorption resin, Sephadex LH-20 and silica gel column chromatography and identified on the basis of their physicochemical and spectral data.