[Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875].

Objective: To study the effect of Bt9875 crystal protein treated with proteinase K on human cancer cells, HL-60.

Methods: We used the methods of Thiazolyl Blue Tetrazolium Bromide, fluorescence microscopy examination, agarose gel electrophores and flow cytometry to detect the growth inhibition rate and apoptosis characters of the HL-60 cells that were treated with different concentration of Bt9875 crystal protein.

Results: Bt9875 crystal protein inhibited the growth of HL-60 cells evidently in a dose-dependent manner, with minimal effects on normal human peripheral blood mononuclear cells (PBMCs).