Sequence analysis of SWEET transporters from trypanosomatids and evaluation of its expression in Trypanosoma cruzi.

Trypanosoma cruzi is an obligate parasite that uses glucose as one of the main resources to maintain its survival and proliferation. In eukaryotic cells glucose transport across membranes is mediated by facilitated transport through a variety of transporters. Herein, genes from the recently described SWEET family of carbohydrate transporters were identified in trypanosomatid parasites, including the medically important species T.

Environmental Surveillance of Polioviruses in Armenia, Colombia before Trivalent Oral Polio Vaccine Cessation.

Although acute flaccid paralysis (AFP) surveillance is the "gold standard" for detecting cases of polio, environmental surveillance can provide supplementary information in the absence of paralytic poliomyelitis cases. This study aimed to detect the introduction and/or circulation of wild poliovirus or vaccine-derived polioviruses (VDPV) in wastewater, covering a significant population of Armenia, Colombia, before trivalent oral polio vaccine (OPV) cessation. Between March and September 2015, 24 wastewater samples were collected from eight study sites in eight communes of Armenia, Colombia.

[Modulation of high-density lipoprotein and cytokine IL-1β and IL-6 levels in patients with dengue].

Objectives: To assess High-Density Lipoprotein (HDL) and pro-inflammatory cytokine levels in patients with dengue classified according to the severity of the clinical presentation.

Materials And Methods: A descriptive study was carried out with 70 individuals with dengue, classified as no alarm signs (DSSA), with signs of alarm (DCSA), and severe dengue (DG); 15 healthy donors were included as controls (CS). The serum levels of IL-1β and IL-6 were quantified through ELISA, and the HDL levels through serum by a colorimetric test.

Identification, Characterization, Immunolocalization, and Biological Activity of Lucilin Peptide.

Maggots from the Lucilia sp. genus are used for debridement of infected and necrotic wounds. Broad-spectrum antimicrobial activity has been described in the excretion/secretions (ES) of these larvae.

Inhibitors compounds of the flavivirus replication process.

Flaviviruses are small viruses with single-stranded RNA, which include the yellow fever virus, dengue virus, West Nile virus, Japanese encephalitis virus, tick-borne encephalitis virus, and Zika virus; and are causal agents of the most important emerging diseases that have no available treatment to date. In recent years, the strategy has focused on the development of replication inhibitors of these viruses designed to act mainly by affecting the activity of enzyme proteins, such as NS3 and NS5, which perform important functions in the viral replication process. This article describes the importance of flaviviruses and the development of molecules used as inhibitors of viral replication in this genus.

[Seroprevalence of antibodies to measles, rubella, mumps, hepatitis B viruses and all three poliovirus serotypes among children in Quindío, Colombia].

Objective The main goal of this research was to assess the seroprevalence of antibodies against measles, rubella, mumps, hepatitis B and all three poliovirus serotypes among children in the Quindío Department, Colombia. Methods Blood samples were obtained from 170 healthy children aged 5-9 years from nine municipalities in Quindío. The presence of serum IgG antibodies against measles, rubella, mumps and Hepatitis B were determined using commercial indirect ELISA kits.

[Outbreak of malaria in an indigenous population living in an urban area of Armenia, Colombia, 2012].

Introduction: Malaria is the most frequent mosquito-borne parasitic infection in the tropical regions of the world. Due to different factors, including climate change, this disease can emerge or reemerge in different areas in the planet.

Objective: To describe an autochthonous outbreak of urban malaria in Armenia, Colombia.

[Prevalence of intestinal helminths in cats in Quindío, Colombia].

Introduction: Diseases caused by helminths are widely distributed in the world and many of them are considered zoonoses in which pets play a major role in transmission to humans.

Objective: The prevalence of intestinal helminths was determined in cats in Quindío Province.

Materials And Methods: One hundred twenty-one cats were characterized --data recorded included sex, age and body condition.

[Effect of maggot therapy on minimally necrotic tissues: characterization of larval enzymatic excretion/secretion].

Introduction: Chronic leg ulcers are a burden for the health system and impact quality of life. The infections, the necrotic tissue and the difficult treatment affects the prognosis and healing time. Maggot therapy is presented as an acceptable alternative for the debridement and treatment of this pathology.

[Absence of poliovirus circulation in Colombian departments with vaccination coverage below 80%].

This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription.

[Prevalence of enterovirus infection in infants in Armenia, Colombia, 2009].

Introduction: Despite world wide circulation of enteroviruses, little information has accumulated on the circulation of enteroviruses in Colombia.

Objective: The prevalence of enterovirus circulation was examined in children under 1 year to identify the most common enterovirus serotypes.

Materials And Methods: Fecal samples were collected from 320 children under 1 year of age who attended a first-level health center in the city of Armenia, Colombia, in 2009.

[Prevalence of Mammomonogamus laryngeus(Strongylida:Syngamidae) nematodes in a bovine slaughterhouse].

Introduction: Mammomonogamus laryngeus is a nematode parasite that is usually found in the larynx of mammals in the tropics, especially in cattle, sheep and cats.

Objective: The prevalence of M. laryngeus was determined in cattle brought to an abattoir as an indicator of its prevalence in the general population.

[First case report of Mammomonogamus (Syngamus) laryngeus human infection in Colombia].

First case report of Mammomonogamus (Syngamus) laryngeus human infection in Colombia Parasitic nematodes of the genus Mammomonogamus affect the respiratory tract of domestic mammals. The male and female of M. laryngeus remain in permanent copula so that the pair appears as a "Y'.

[Detecting poliovirus in wastewater from Armenia, Colombia].

Objectives: Implementing environmental surveillance for poliovirus circulating from wastewater.

Methods: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent.

[Prevalence of intestinal helminths in dogs from Quindío Province].

Background: Intestinal helminths are pathogens for domestic animals and provide a source of potential infection for humans.

Objectives: The prevalence of intestinal helminths in domestic dogs was determined in a province-wide survey in Quindío Province, Colombia.

Materials And Methods: The sample size was calculated based upon the data of the 2003 antirabies vaccination program in Quindio.

[Molecular and biological characterization of the CIBMUQ/HDC strain, a reference strain for Colombian Toxoplasma gondii].

There are few reports about characterization strains of Toxoplasma gondii that analyze the differences between isolates from Europe or United States with those obtained in South America. The current study analyzes virulence data from the mouse model, the gene SAG2 polymorphism by PCR-RFLP and microsatellite analysis in a single Colombian isolate. The strain was isolated from blood of a child with congenital toxoplasmosis, living in Armenia, Colombia.