MPH-GST sensing microplate for easy detection of organophosphate insecticides.

Objective: To develop a convenient and efficient means for organophosphate (OP) insecticide detection, a simple, cost-effective, and easy-to-use absorbance-based sensing device was generated using methyl parathion hydrolase fused with glutathione-S-transferase (MPH-GST) covalently immobilized onto a chitosan film-coated microplate.

Results: With methyl parathion (MP) as a representative substrate, this MPH-GST sensing microplate had the detection limit of 0.1 µM and the linear range of 0.

Easy-to-use and reliable absorbance-based MPH-GST biosensor for the detection of methyl parathion pesticide.

Due to high contamination of organophosphate (OP) insecticides in agricultural products and the environment, efficient and convenient devices for their monitoring are necessary. Here, a simple, inexpensive, efficient, and easy-to-use absorbance-based biosensor was fabricated utilizing recombinant methyl parathion hydrolase fused with glutathione--transferase (MPH-GST), covalently immobilized onto a chitosan film-coated polystyrene microplate, for the detection of methyl parathion (MP) as a representative of OPs. Having been connected to the transducer system designed to work through an Arduino microcontroller, the biosensor could detect MP as efficiently as the conventional methods, with the detection limit of 0.