Predictive Significance of Glycosyltransferase-Related lncRNAs in Endometrial Cancer: A Comprehensive Analysis and Experimental Validation.

Glycosylation is a crucial post-translational modification that regulates cellular functions and the surrounding microenvironment. The abnormal expression of glycosyltransferases, essential enzymes in this process, has been linked to tumor development. In addition, long noncoding RNAs (lncRNAs) have been shown to exert a multifaceted regulatory influence on gene expression.

Distinct Clinical Phenotypes in KIF1A-Associated Neurological Disorders Result from Different Amino Acid Substitutions at the Same Residue in KIF1A.

KIF1A is a neuron-specific kinesin motor responsible for intracellular transport along axons. Pathogenic mutations cause KIF1A-associated neurological disorders (KAND), a spectrum of severe neurodevelopmental and neurodegenerative conditions. While individual mutations have been studied, how different substitutions at the same residue affect motor function and disease progression remains unclear.

Unveiling the Complete Spectrum of SARS-CoV-2 Fusion Stages by In Situ Cryo-ET.

SARS-CoV-2 entry into host cells is mediated by the spike protein, which drives membrane fusion. While cryo-EM has revealed stable prefusion and postfusion conformations of the spike, the transient intermediate states during the fusion process have remained poorly understood. Here, we designed a near-native viral fusion system that recapitulates SARS- CoV-2 entry and used cryo-electron tomography (cryo-ET) to capture fusion intermediates leading to complete fusion.

Genomic factors limiting the diversity of Saccharomycotina plant pathogens.

The Saccharomycotina fungi have evolved to inhabit a vast diversity of habitats over their 400-million-year evolution. There are, however, only a few known fungal pathogens of plants in this subphylum, primarily belonging to the genera and . We compared the genomes of 12 plant-pathogenic Saccharomycotina strains to 360 plant-associated strains to identify features unique to the phytopathogens.

Functional retrogression of LOFSEPs in specifying floral organs in barley.

Unlabelled: The barley genome encodes a complete set of MADS-box proteins sharing homology with components of the ABCDE model, which explains the molecular basis of floral organ identity in angiosperm flowers. Although the E-class members are universally expressed across floral whorls and crucial for flower development in Arabidopsis and rice, the functional role of the barley E-class LOFSEP subfamily (comprising MADS1, MADS5, and MADS34) remains elusive, particularly during spikelet formation. Here, we characterize the single, double and triple mutants in barley in an attempt to overcome the anticipated genetic redundancy.

Curcumol promotes ferroptosis of colon cancer by targeting the ubiquitination and degradation of GPX4.

Background And Aim: Colon cancer (CC) is one of the common malignant tumors in the digestive tract, the prognosis of CC patients has never been satisfying. A Ferrous-dependent form that regulates cell death, plays a key role in cancer development. As a core regulator of ferroptosis, GPX4 has become a potential molecular target for the development of antitumor drugs.

Primary and Re-exposure effects of D-enantiomeric peptide on metabolism, diversity, and composition of oral biofilms at different stages of recovery.

The persistence of bacteria in the root canal system is the primary cause of recurrent apical periodontitis. The adaptability of residual bacteria to changing environmental conditions is a key survival strategy of biofilms, often leading to endodontic treatment failure. DJK-5 is a protease-resistant, broad-spectrum D-enantiomeric peptide that degrades or prevents the accumulation of guanosine penta- and tetraphosphates, which are important for biofilm formation.

Characteristics of Gut Microbiota and Plasma Metabolites in Patients with Post-Stroke Depression.

Purpose: The changes in gut microbiota and plasma metabolites have been proposed to play a key role in post stroke depression (PSD), but clinical study based on combined omics is still in lack. This study aimed to investigate the characteristics of gut microbiota and plasma metabolites in patients 3 months after the onset of acute ischemic stroke (AIS), compare PSD and non-PSD groups, and explore possible diagnostic biomarkers.

Patients And Methods: Seventy patients with stroke were included at 3 months after AIS onset.

Distribution of Angle Alpha in a Large Population in Eastern China: An Analysis of the 30705 Eyes Using the Ray Tracing Aberrometer.

Purpose: To describe the distribution of angle alpha orientation and magnitude in a large population and to analyze the impact of eye laterality, gender, and age on angle alpha.

Setting: EENT Hospital of Fudan University and Shanghai Heping Eye Hospital, 2017-2023.

Design: A retrospective analysis utilizing the iTrace system to examine preoperative angle alpha in patients undergoing cataract surgery.

Identification of Anoikis-Related Genes in Driving Immune-Inflammatory Responses in Ulcerative Colitis Based on Bioinformatics Analysis and Machine Learning.

Background: Ulcerative colitis (UC) is a challenging chronic intestinal inflammation. Anoikis, a type of programmed cell death triggered by detachment from the extracellular matrix, is crucial in various physiological and pathological contexts. This study aims to explore the biological and clinical implications of anoikis-related genes (ARGs) in UC.

Pseudomembranous Aspergillus tracheobronchitis: A Rare Manifestation of Invasive Aspergillosis in a Immunocompetent Patient.

Purpose: Pseudomembranous Aspergillus tracheobronchitis (PMATB), an uncommon clinical form of invasive aspergillosis, is mainly occurs in patients who are moderate to severely immunocompromised. There are some case reports of immunocompetent individuals developing invasive aspergillosis after occupational exposure (Primarily observed in farmers, sawmill workers, waste collectors, mushroom processing workers, or those who handle grain, hay, or straw), including allergic bronchopulmonary aspergillosis (ABPA), severe asthma with fungal sensitization, hypersensitivity pneumonitis, and invasive pulmonary aspergillosis. To our knowledge, there are no published case reports in the literature with PMATB as the main presentation in construction worker after occupational exposure.

SUV39H1 maintains cancer stem cell chromatin state and properties in glioblastoma.

Glioblastoma (GBM) is the most lethal brain cancer, with GBM stem cells (GSCs) driving therapeutic resistance and recurrence. Targeting GSCs offers a promising strategy for preventing tumor relapse and improving outcomes. We identify SUV39H1, a histone-3, lysine-9 methyltransferase, as critical for GSC maintenance and GBM progression.

Metabolic shifts in tryptophan pathways during acute pancreatitis infections.

Infectious complications (ICs) in acute pancreatitis (AP) are primarily driven by intestinal bacterial translocation, significantly increasing mortality and hospital stays. Despite this, the role of the gut microenvironment, particularly its metabolic aspects, in AP remains poorly understood. In this study, we investigated a cohort of patients with AP, and conducted supplemental murine studies, to explore the relationship between the gut metabolome and the development of ICs.

Synergistic immunomodulatory effect of wheat/soybean/sea cucumber peptides in chloramphenicol induced immunosuppression zebrafish.

Background: Food-derived immunomodulatory peptides serve as vital agents in promoting health by protecting the body against pathogens. The present study focused on determining the optimal ratio for combining different peptides to form a complex that enhances immune activity in immunosuppressed zebrafish.

Results: We established a method for immunosuppression in zebrafish, discovering that treatment with 125 μg mL chloramphenicol significantly decreased the macrophage number and neutrophil fluorescence intensity.

Improved Modeling of Vegetation Phenology Using Soil Enthalpy.

Many vegetation phenological models predominantly rely on temperature, overlooking the critical roles of water availability and soil characteristics. This limitation significantly impacts the accuracy of phenological projections, particularly in water-limited ecosystems. We proposed a new approach incorporating soil enthalpy-a comprehensive metric integrating soil moisture, temperature, and texture-to improve phenological modeling.

Synthesis of a Novel DMSP-Typed Zwitterion and Construction on PET Surface via Surface-Initiated RAFT Method.

Mimicked by the structure of dimethyl sulfoxide propionate (DMSP), a novel zwitterion monomer of -methylacryloyl -methyl l-cysteine methyl sulfonium salt (NMASMCMS) was synthesized and characterized for the first time through three steps using l-cysteine as a starting material. Poly(NMASMCMS) brushes were constructed on PET sheets via surface-initiated reversible addition-fragmentation chain-transfer (SI-RAFT) polymerization. The physical and chemical structures were characterized by water contact angle (WCA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM).

Design, synthesis, biological evaluation, and X-ray single crystal structure of novel computer-aided-drug-designbased α-mangostin derivatives: Multifunctional ligands against Alzheimer's disease.

Multifunctional ligand design strategy may be a promising approach for the treatment of Alzheimer's disease (AD). α-Mangostin (α-M), a natural small molecule with anti-AD properties, was used as the lead compound for the design and synthesis of six α-M derivatives (1-6) with the help of computer-aided-drug-design (CADD). Both theoretical calculations and experimental results suggested that 1-6 might serve as promising selective butyrylcholinesterase (BuChE) inhibitors and amyloid-β (Aβ) aggregation inhibitors.

Mobile Oxygen Capture Enhances Photothermal Stability of Perovskite Solar Cells Under ISOS Protocols.

Stability testing protocols from the International Summit on Organic and Hybrid Solar Cell Stability (ISOS) are essential for standardizing studies on the photothermally operational stability of perovskite solar cells (PSCs). Under photothermal conditions, the migration of oxygen from SnO layer induces cationic dehydrogenation at the A-site of the perovskite, accelerating degradation to PbI. This leads to the formation of photoinduced I and Pb defects, significantly compromising long-term stability.

Programmable Non-Volatile Photonic Analog-to-Digital Converter Based on Back-End-of-Line Compatible Phase-Change Materials.

High-performance signal processing and telecommunication systems absolutely necessitate analog-to-digital converters (ADCs) that offer extensive bandwidth, exceptional precision, and minimal power consumption, in order to efficiently convert real-world analog signals into digital signals. While current electronic ADCs are constrained by limitations such as low bandwidth, high jitter noise, susceptibility to electromagnetic interference, and excessive energy consumption, photonic ADCs present promising solutions to overcome these challenges. Here, a programmable photonic ADC is developed by integrating phase-change materials (PCMs) with silicon photonics fabricated using foundry processes.

The Impact of Indoor Environments on the Abundance of Urban Outdoor VOCs.

With the upcoming transition to clean electric vehicles, the sources of volatile organic compounds (VOCs) in the ambient environment are rapidly changing and highly uncertain. Here, through systematic characterization of emissions from a typical apartment in a Chinese megacity (Shenzhen), we show that indoor environments contribute significantly to the levels of ambient (i.e.

Toward Practical All-Solid-State Batteries: Current Status of Functional Binders.

All-solid-state batteries (ASSBs) are promising candidates for next-generation energy storage devices due to their high energy density and enhanced safety. Binder plays an irreplaceable role in stabilizing the electrode structure, enhancing carrier transport and modulating solid electrolyte interfaces by connecting each component of the electrode. The development of functional binders is seen as a key strategy to achieve higher energy densities of ASSBs.

Boron-Doped Graphene as Anion-Trapping Reagent in Poly(ethylene oxide)-Based Solid-State Electrolyte.

Although many protocols have been developed to enhance the ionic conductivity and lithium-ion transference numbers of the solid polymer electrolyte, it is still challenging to improve them simultaneously. Herein, we design and prepare boron-doped graphene (BG) as an anion trapper and blend it within a poly(ethylene oxide) (PEO)-based electrolyte. The well-dispersed BG sheets can reduce the crystallinity of PEO and afford numerous Lewis acid sites to effectively accelerate the dissociation of the lithium salt and trap the anions.

Robust Spatial Cell-Type Deconvolution with Qualitative Reference for Spatial Transcriptomics.

Many spatially resolved transcriptomic technologies have been developed to provide gene expression profiles for spots that may contain heterogeneous mixtures of cells. To decompose cellular composition and expression levels, various deconvolution methods have been developed using single-cell RNA sequencing (scRNA-seq) data with known cell-type labels as a reference. However, in the absence of a reliable reference dataset or in the presence of heterogeneous batch effects, these methods may introduce bias.

TMSOTf/TfOH-Promoted [4 + 2] Annulation of Ynamides with 2-Aminoarylnitriles To Construct 2,4-Diaminoquinolines.

An effective strategy to synthesize 2,4-diaminoquinoline compounds has been efficaciously developed via a TMSOTf/TfOH-promoted [4 + 2] annulation of ynamides with 2-aminoarylnitriles. Compared with the reported transition-metal catalysts, this metal-free promotion system presented a remarkable advancement, enabling the facile and regiospecific assembly of 2,4-diaminoquinoline frameworks with wide functional group compatibility and moderate to excellent yields.

Comparative risk of QTc prolongation induced by second-generation antipsychotics in the real world: retrospective cohort study based on a hospital information system.

Background: Second-generation antipsychotics (SGAs) can cause corrected QT interval (QTc) prolongation as a side-effect. This may limit their clinical use and pose safety concerns for patients.

Aims: To analyse the risk of QTc prolongation associated with eight second-generation antipsychotics and observe the timing characteristics of QTc prolongation events and subsequent changes in medication strategies.