Metal-Free Electrochemical C-H Chlorination of Terminal Alkanes.

Although research on the activation of C-H bonds in alkanes has been ongoing for decades, there are still few strategies that are both highly selective and suitable for industrial production. Here, we report a highly selective method for chlorination of terminal C-H bonds in alkanes by combining electrochemistry and organocatalysis. The specific cavity size of organic molecular catalysts ensures high regioselectivity, while the use of inexpensive and easily reusable graphite felt electrodes, simple electrochemical device and mild conditions enables the reaction to maintain good efficiency even when scaled up to kilogram level production.

Insight into the Specific Adsorption of Cu(II) by a Zinc-Based Metal-Organic Framework Mediated via a Proton-Exchange Mechanism.

In the context of scarce metal resources, the one-step separation and recovery of high-value copper metal ions from secondary resources is of significant importance and presents substantial challenges. This study identified a Zn-based triazole MOF (Zn(tr)(OAc)) with accessible and noncoordinated terminal hydroxyl groups within its framework. The Zn(tr)(OAc) surpasses most currently reported Cu-specific MOF adsorbents regarding adsorption capacity and Cu selectivity.

Inducible regulating homologous recombination enables precise genome editing in Pichia pastoris without perturbing cellular fitness.

The methylotrophic yeast Pichia pastoris (also known as Komagataella pastoris) is an ideal host for producing proteins and natural products. Enhancing homologous recombination (HR) is helpful for improving the precision of genome editing, but results in stress to cellular fitness and is harmful for industrial applications. To overcome these challenges, we developed a tetracycline repressor protein (TetR)/tetO2 inducible system to dynamically regulate the HR-related gene RAD52 in P.

Twists, turns and jumps: T6SS evolution within species.

The type VI secretion system mediates interbacterial antagonism between Gram-negative bacteria through delivery of toxic effector proteins. A recent comprehensive genomic analysis by Habich et al. reveals interesting features of the evolution of T6SSs and their corresponding effectors in Pseudomonas aeruginosa, raising questions about functional specialization of the system.

Phosphating CoMoO-Modified Hematite-Based Photoanode Enhances Surface Charge Transfer and Reaction Activity for Efficient Photoelectrochemical Water Oxidation.

The photoelectrochemical properties of hematite-based photoanodes are hindered by severe carrier recombination and poor reaction activity, which is a major challenge. Herein, we coupled zirconium-doped α-FeO (Zr:FeO) and phosphating cobalt molybdate electrocatalyst (P-CoMoO) to ameliorate the above difficulties. The conductivity and carrier density of hematite significantly increase by Zr doping.

Wearable device-measured physical activity and incident cardiovascular disease in cancer survivors.

Objective: To explore the association of wearable device-measured moderate-to-vigorous intensity physical activity (MVPA) with cardiovascular disease (CVD) risk in long-term cancer survivors.

Methods: This retrospective analysis involved a prospective cohort of 6109 cancer survivors without CVD from the UK Biobank accelerometry subsample. The MVPA volume is categorised into four groups based on guideline recommendations (0-75 min/week, 75-150 min/week, 150-300 min/week, ≥300 min/week).

BEL1-like homeodomain transcription factor SAWTOOTH1 (MdSAW1) in Malus domestica enhances the tolerance of transgenic apple and Arabidopsis to zinc excess stress.

In recent years, the phenomenon of zinc pollution in orchards has become increasingly serious, and the safety of apple production is facing a major risk. Therefore, exploring excellent genes for zinc tolerance has a positive effect on apples. Up to now, there is still a lack of attention on genes related to zinc stress tolerance in apples.

Explore peptides extracted from gliadin hydrolysates suppressing BACE1 activity and restraining Aβ protein deposition.

Alzheimer's Disease (AD) constitutes approximately 70 % of dementia cases and is the most prevalent form of dementia. Current therapeutic options, including acetylcholinesterase inhibitors and N-methyl d-aspartate (NMDA) receptor antagonists, provide symptomatic relief but do not cure the disease and often come with side effects. The primary pathological features of AD are amyloid plaques and neurofibrillary tangles, with amyloid plaques formed by the abnormal accumulation of Amyloid-β (Aβ).

Oxidative stress markers predict treatment outcomes in patients with generalized anxiety disorder treated with selective serotonin reuptake inhibitors.

Introduction: The etiology of generalized anxiety disorder (GAD) has not been fully understood, and oxidative stress may potentially contribute to its pathogenesis. However, there is no published evidence concerning the possible influence of oxidative stress on antidepressant treatment outcomes. This study investigated the ability of oxidative stress markers to predict treatment outcomes in GAD patients treated with selective serotonin reuptake inhibitors (SSRIs).

Effect of Early Tumor Shrinkage and Depth of Response on the Clinical Outcomes of Patients with Unresectable Hepatocellular Carcinoma Treated with Transcatheter Arterial Chemoembolization and Lenvatinib Plus PD-1 Inhibitors.

Introduction: Systematic therapies, including tyrosine kinase inhibitors (TKIs) and immune checkpoint inhibitors (ICIs), have now been approved as the mainstay treatment for patients with unresectable hepatocellular carcinoma (uHCC). However, only a minority of the patients are expected to respond to TKIs and ICIs. Because early tumor shrinkage (ETS) and depth of response (DoR) might have the potential to predict survival outcomes, this study aimed to identify the optimal cutoffs for ETS and DoR to predict patients' clinical outcomes in their early treatment stage.

Lactobacillus reuteri alleviates diquat induced hepatic impairment and mitochondrial dysfunction via activation of the Nrf2 antioxidant system and suppression of NF-κB inflammatory response.

Accumulating evidence has shown that elevated oxidative stress and inflammatory response leads to hepatic impairment and dysfunction of hens during the aging process. This study was conducted to investigate the potential regulatory mechanisms of Lactobacillus reuteri (L. reuteri) in alleviating hepatic oxidative stress and dysfunction induced by diquat (DQ) exposure.

Thermal Inactivation of Salmonella Surrogate, Enterococcus faecium, in mash broiler feed pelleted in a university pilot feed mill: Microbiology and Food Safety Section.

This study evaluated the thermal inactivation kinetic parameters of a Salmonella surrogate Enterococcus faecium (E. faecium) during feed manufacture in a university pilot feed mill setting. A batch of 227 kg mash broiler feed was pelleted after being inoculated with 1,000 mL of nalidixic acid (NaL) resistant E.

A multifunctional semi-interpenetrating polymer network hydrogel dressing for wound healing.

Hydrogels have exhibited significant application potential in the field of new wound dressings due to their unique physicochemical properties and biological functions. However, traditional hydrogels possess limitations regarding mechanical properties, adhesion, and the promotion of wound healing. Herein, a multifunctional polyvinyl alcohol-tannic acid/polyacrylamide-polydopamine (PVA-TA/PAM-PDA) hydrogels are developed.

In vitro hydrogen production by mammal [FeFe]-hydrogenase-like protein.

Although emerging evidence in mammals reveals that exogenously applied H positively regulates numerous physiological and pathological responses, it remains unclear whether and how mammalian cells produce H endogenously. Here, we report for the first time that recombinant human (Homo sapiens) and pig (Sus scrofa) nuclear prelamin recognition factor (Narf)-like proteins (also known as H. sapiens iron-only hydrogenase-like protein 1 [HsIOP1] and S.

Strategic crossing decisions in vehicle interactions at uncontrolled intersections: A networked driving simulator study.

At uncontrolled intersections, without traffic signals or explicit right-of-way guidance, drivers interacting from different directions depend on their perception and real-time judgment to make strategic decisions about when and how to cross or yield. This uncertainty in decision-making, if misjudged, may result in vehicle collisions. In this study, we developed a networked two-vehicle driving simulator platform, conducted experiments to simulate the interaction between straight-going (SG) and left-turn (LT) vehicles at uncontrolled intersections, and focused on exploring the contributing factors that may influence drivers' crossing decisions.

The transcription factor GmbZIP131 enhances soybean salt tolerance by regulating flavonoid biosynthesis.

Detoxifying reactive oxygen species (ROS) that accumulate under saline conditions is crucial for plant salt tolerance. The Salt Overly Sensitive (SOS) pathway functions upstream, while flavonoids act downstream, in ROS scavenging under salt stress. However, the potential crosstalk between the SOS pathway and flavonoids in regulating salt stress responses and the associated mechanisms remain largely unexplored.

CAD manipulates tumor intrinsic DHO/UBE4B/NF-κB pathway and fuels macrophage cross-talk, promoting hepatocellular carcinoma metastasis.

Background And Aims: Portal vein tumor thrombosis (PVTT), an indicator of clinical metastasis, significantly shortens hepatocellular carcinoma (HCC) patients' lifespan, and no effective treatment has been established. We aimed to illustrate mechanisms underlying PVTT formation and tumor metastasis, and identified potential targets for clinical intervention.

Approach And Results: Multi-omics data of 159 HCC patients (including 37 cases with PVTT) was analyzed to identify contributors to PVTT formation and tumor metastasis.

Upper Arm Versus Forearm Placement of Long Peripheral Catheters for Blood Sampling: A Randomized Controlled Trial.

Background: The impact of site selection on blood sampling and catheter functionality for long peripheral catheters (LPCs) is unclear.

Purpose: To compare outcomes of LPCs placed in the upper arm vs the forearm.

Methods: A single-site, randomized trial was conducted among adult patients requiring an LPC for difficult venous access or prolonged therapy.

6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins.

6-methyladnosine (mA) modification is present in both positive- and negative-strand RNA of influenza A virus (IAV) and affects the replication and pathogenicity of IAV. However, little is known about the regulatory mechanism of mA in IAV RNA. In the present study, we identified the mA methylation of the viral RNA of different IAV subtypes and confirmed that mA modification promotes the polymerase activity and replication of IAV.

Proteasomal processing of the viral replicase ORF1 facilitates HEV-induced liver fibrosis.

Chronic infections with hepatitis E virus (HEV), especially those of genotype 3 (G3), frequently lead to liver fibrosis and cirrhosis in patients. However, the causation and mechanism of liver fibrosis triggered by chronic HEV infection remain poorly understood. Here, we found that the viral multiple-domain replicase (ORF1) undergoes unique ubiquitin-proteasomal processing leading to formation of the EV-erived MAD ctivator (HDSA), a viral polypeptide lacking putative helicase and RNA polymerase domains.

Correction: The association of mental health with physical activity and its dimensions in Chinese adults: A cross-sectional study.

[This corrects the article DOI: 10.1371/journal.pone.

A rhizobacterium-secreted protein induces lateral root development through the IAA34-PUCHI pathway.

Lateral roots (LRs) can continuously forage water and nutrients from soil. In Arabidopsis thaliana, LR development depends on a canonical auxin signaling pathway involving the core transcription factors INDOLE-3-ACETIC ACIDs (IAAs) and AUXIN RESPONSE FACTORs (ARFs). In this study, we identified a protein, bacillolysin, secreted by the beneficial rhizobacterium Bacillus velezensis SQR9, that is able to stimulate LR formation of Arabidopsis.

Mesh-reinforced reconstruction after abdominal wall desmoid tumor resection: a retrospective analysis of 16 cases.

Purpose: Managing the defect after abdominal wall desmoid tumor resection is challenging due to the wide excision required. This report aims to review our institutional experience with mesh-reinforced reconstruction following desmoid tumor resection in the abdominal wall.

Methods: We retrospectively reviewed patients who underwent abdominal wall desmoid tumor resection with mesh-reinforced reconstruction between April 2014 and January 2019.

Nomogram predicts risk score and likehood of orchiectomy in patients with testicular torsion: a multicenter retrospective study.

Purpose: Testicular torsion is a prevalent scrotal emergency associated with a significant risk of testicular loss, and there is no reliable scoring system available to assess the likelihood of orchiectomy following testicular torsion.

Methods: Clinical characteristics and surgical data of patients with testicular torsion from January 2015 to May 2024 were gathered from National Center for Children's Health (NCCH) and the Children's Hospital of Capital Institute of Pediatrics (CIPCH). Independent risk variables for testicular resection were evaluated by logistic regression analyses, leading to the establishment of a nomogram.