Towards stimulated Raman scattering spectro-microscopy across the entire Raman active region using a multiple-plate continuum.

Stimulated Raman scattering (SRS) has attracted increasing attention in bio-imaging because of the ability toward background-free molecular-specific acquisitions without fluorescence labeling. Nevertheless, the corresponding sensitivity and specificity remain far behind those of fluorescence techniques. Here, we demonstrate SRS spectro-microscopy driven by a multiple-plate continuum (MPC), whose octave-spanning bandwidth (600-1300 nm) and high spectral energy density (∼1 nJ/cm) enable spectroscopic interrogation across the entire Raman active region (0-4000 cm), SRS imaging of a Drosophila brain, and electronic pre-resonance (EPR) detection of a fluorescent dye.

Greater than 50 times compression of 1030 nm Yb:KGW laser pulses to single-cycle duration.

Generation of octave-spanning spectrum that spans from 570 nm to 1300 nm utilizing 1030 nm 170 fs pulses from a Yb:KGW laser and a two-stage multiple-plate arrangement is demonstrated. 3.21 fs sub-single-cycle pulses are obtained after dispersion compensation.