In vitro transduction of antimicrobial resistance genes into isolates from backyard poultry in Mexico.

The transmission of multidrug-resistant pathogens and antimicrobial resistance genes is an emerging problem involving multiple factors (humans, domestic animals, wildlife). The aim of this study was to investigate the presence of isolates with different antimicrobial resistance genes from backyard poultry and to demonstrate the in vitro transduction phenomenon of these genes between phages from migratory wild birds and poultry isolates. We collected 197 isolates from chickens, turkeys, and ducks in backyard production units (northern region of the State of Mexico).

Determination of extended spectrum β-lactamases/AmpC β-lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico.

Food-borne bacterial infections have worldwide importance, and a great variety of antibiotic resistance mechanisms, mainly of the chromosome type, have rapidly developed. Antimicrobial resistance was determined in this study in terms of the presence of extended-spectrum β-lactamases (ESBLs), plasmid AmpC β-lactamases (pAmpC), and plasmid-mediated quinolone resistance (PMQR) from 155 Escherichia coli isolates obtained from bovine carcasses from two states in Mexico (states of Mexico and Jalisco). Isolates were challenged with β-lactam antimicrobials (ampicillin, ceftazidime, and cefotaxime) and quinolones (nalidixic acid and ciprofloxacin).