Complete Genome Sequences of Four Mycobacteriophages Involved in Directed Evolution against Undisputed Clinical Strains.

Phage therapy is still in its infancy, but it is increasingly promising as a future alternative for treating antibiotic-resistant bacteria. To investigate the effect of phages on complex (MABC), we isolated 113 environmental phages, grown them to high titres, and assayed them on MABC clinical strains through the spot test. Of all the phages, only 16 showed killing activity.

Risk factors for poor health and performance in European broiler production systems.

Background: Conventional broilers are currently one of the most efficient protein converters. Although decades of progress in genetic selection and feed formulation have lead to high standards of efficient broiler production, still a lot of variability is found between farms and between successive flocks. The aim of this study was to investigate risk- and/or protective factors for poor health and performance in conventional broiler-farms in Europe by developing eight multivariable linear mixed models.

Alcohol and Road Traffic Injuries in Latin America and the Caribbean: A Case-Crossover Study.

Background: This study reports dose-response estimates for the odds ratio (OR) and population attributable risk of acute alcohol use and road traffic injury (RTI).

Methods: Data were analyzed on 1,119 RTI patients arriving at 16 emergency departments (EDs) in Argentina, Brazil, Costa Rica, Dominican Republic, Guatemala, Guyana, Mexico, Nicaragua, Panama, and Trinidad and Tobago. Case-crossover analysis, pair-matching the number of standard drinks consumed within the 6 hours prior to the RTI with 2 control periods (prior d/wk), was performed using fractional polynomial analysis for dose-response.

Optimization of antimicrobial combined effect of organic acids and temperature on foodborne Salmonella and Escherichia coli in cattle feed by response surface methodology.

Despite the importance of feedstuffs and feed ingredients in the food chain, few studies have analyzed the effectiveness of usual methods for the microbial decontamination of feeds. This work aimed to study the combined effect of temperature and organic acids (formic or lactic) on the inactivation of 10 isolates of Salmonella enterica and Escherichia coli obtained from vegetable feed ingredients, including cereals and other processed materials, in cattle feed. A central composite design was used with acid concentrations ranging from 0 to 0.

Effects of feeding of two potentially probiotic preparations from lactic acid bacteria on the performance and faecal microflora of broiler chickens.

The aim of this study was to evaluate the potential of two probiotic preparations, containing live lactic acid bacteria (Lactococcus lactis CECT 539 and Lactobacillus casei CECT 4043) and their products of fermentation (organic acids and bacteriocins), as a replacement for antibiotics in stimulating health and growth of broiler chickens. The effects of the supplementation of both preparations (with proven probiotic effect in weaned piglets) and an antibiotic (avilamycin) on body weight gain (BWG), feed intake (FI), feed consumption efficiency (FCE), relative intestinal weight, and intestinal microbiota counts were studied in 1-day posthatch chickens. The experiments were conducted with medium-growth Sasso X44 chickens housed in cages and with nutritional stressed Ross 308 broiler distributed in pens.

Supplementation with plant extracts (carvacrol, cinnamaldehyde and capsaicin): its effects on acid-base status and productive performance in growing/finishing bull calves.

This study investigated the in vivo effects of a commercial blend of plant extracts (carvacrol, cinnamaldehyde and capsaicin) on blood acid-base balance and serum lactate levels in a 148-day feedlot experimentwith 24 double-muscled Belgian Blue bull calves. Animals were allotted randomly to one of two experimental groups: 1) a control group (C, no supplementation; n = 10), and 2) a group receiving dietary supplementation with a combination of plant extracts (PE, 100 mg per kg DM of concentrate; n = 14). All animals received a high-grain ration, typical of diets fed commercially to feedlot cattle in Spain, consisting mainly of barley plus other components in proportions depending on the production phase.

Effects of malate supplementation on acid-base balance and productive performance in growing/finishing bull calves fed a high-grain diet.

This study investigated the effects of malate supplementation on blood acid-base balance and serum lactate levels in a 137-day feedlot experiment with bull calves. Animals were allotted to one of two experimental groups: (1) A control group (no supplementation), and (2) a group receiving a salt of DL-malic acid. Blood pH, pCO2, HCO3-, base excess, serum L-lactate and productivity parameters were evaluated.

Effects of long-term dietary supplementation of monensin or saccharomyces cerevisiae on blood acid-base and productive performance in growing feedlot steers.

The aim of this study was to evaluate the effects of two dietary supplements (monensin and a live yeast culture) on acid-base balance in steers maintained in a commercial feedlot system, considering effects over the growing period (14 to 23 weeks of age). A 63-day feedlot study was performed using 42 double-muscled Belgian Blue steers. Steers were allotted randomly to one of the three study groups: (1) control group [no supplementation, C], (2) monensin supplementation [MON] at a concentration of 30 mg/kg (DM basis), and (3) live Saccharomyces cerevisiae strain supplementation [SACC] at a dose of 500 mg/kg (DM basis).

Indocyanine green angiography for the detection of subretinal nematodes in diffuse unilateral subacute neuroretinitis (DUSN).

Purpose: Observational case-report on the clinical, fluorescein and indocyanine green angiographic findings in a patient with a presumed subretinal nematode.

Methods: Retinography, angiography with sodium fluorescein and Indocyanine green were performed in a 45 year old man who presented with a neurosensory macular detachment. The lesion studied with fluorescein angiography was atypical for a choroidal neovascular membrane but suggestive of ocular cysticercosis.

Color quality of pigments in cochineals (Dactylopius coccus Costa). Geographical origin characterization using multivariate statistical analysis.

The commercial value of a cochineal (Dactylopius coccus Costa) sample is associated with its color quality. Because the cochineal is a legal food colorant, its color quality is generally understood as its pigment content. Simply put, the higher this content, the more valuable the sample is to the market.

Optimizing conditions for the extraction of pigments in cochineals (Dactylopius coccus Costa) using response surface methodology.

A simple method was developed for the extraction and determination of color pigments in cochineals (Dactylopius coccus Costa). The procedure was based on the solvent extraction of pigments in insect samples using methanol:water (65:35, v:v) as extractant. Two-level factorial design was used in order to optimize the solvent extraction parameters: temperature, time, methanol concentration in the extractant mixture, and the number of extractions.