Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.

Cutaneous Leishmaniasis (CL) is a tropical disease characterized by cutaneous ulcers, sometimes with satellite lesions and nodular lymphangitis. Leishmania parasites, transmitted by sandfly vectors, cause this widespread public health challenge affecting millions worldwide. CL's complexity stems from diverse Leishmania species and intricate host interactions.

Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein.

Background: Leishmaniasis is a parasitic disease caused by obligate intracellular protozoa of the genus Leishmania. This infection is characterized by a wide range of clinical manifestations, with symptoms greatly dependent on the causal parasitic species. Here we present the design and application of a new 70-kDa heat shock protein gene (hsp70)-based marker of 771 bp (HSP70-Long).

Severe mucosal leishmaniasis with torpid and fatal evolution.

Mucosal leishmaniasis is a clinical condition that is difficult to diagnose and treat and usually precedes a cutaneous leishmaniasis condition with a long latency period as observed in our study of a patient who experienced a torpid evolution in 9 months, caused by having had cutaneous leishmaniasis on the neck without therapeutic treatment, although with ulcer closure 18 years earlier, incomplete treatment with antimonials and amphotericin B, with the destruction of the eyeball, a large area of necrosis on the face and nasal bone exposure. Additionally, the patient had chronic anemia (9.4 g/dl), lymphopenia and neutrophilia (lymphocytes 13.

Development of an Amplicon-Based Next-Generation Sequencing Protocol to Identify Species and Other Trypanosomatids in Leishmaniasis Endemic Areas.

Trypanosomatid infections are an important public health threat affecting many low-income countries across the tropics, particularly in the Americas. Trypanosomatids can infect many vertebrate, invertebrate, and plant species and play an important role as human pathogens. Among these clinically relevant pathogens are species from the genera and .

Microbial Communities' Characterization in Urban Recreational Surface Waters Using Next Generation Sequencing.

Microbial communities in surface waters used for recreational purposes are indicators of contamination and risk of contact with human pathogens. Hence, monitoring microbial communities in recreational waters is important for potential public health threats to humans. Such monitoring is rare in Colombia, even in its capital, Bogotá, the most populous city in the country.

SARS-CoV-2 spread across the Colombian-Venezuelan border.

Introduction: Venezuela and Colombia both adopted measures of containment early in response to the COVID-19 pandemic. However, Venezuela's ongoing humanitarian crisis has decimated its health care system, and forced millions of Venezuelans to flee through its porous border with Colombia. The extensive shared border, and illegal cross-border transit through improvised trails between the two countries are major challenges for public health authorities.

A summary of Blastocystis subtypes in North and South America.

Background: Blastocystis is a stramenopile of worldwide significance due to its capacity to colonize several hosts. Based on its high level of genetic diversity, Blastocystis is classified into global ribosomal subtypes (STs). The aim of this study was to conduct a summary of Blastocystis STs and depict their distribution throughout North and South America; we did this by assembling maps and identifying its most common 18S alleles based on diverse studies that had been reported all over the continent and whose Blastocystis-positive samples were obtained from numerous hosts.

A systematic review of the Trypanosoma cruzi genetic heterogeneity, host immune response and genetic factors as plausible drivers of chronic chagasic cardiomyopathy.

Chagas disease is a complex tropical pathology caused by the kinetoplastid Trypanosoma cruzi. This parasite displays massive genetic diversity and has been classified by international consensus in at least six Discrete Typing Units (DTUs) that are broadly distributed in the American continent. The main clinical manifestation of the disease is the chronic chagasic cardiomyopathy (CCC) that is lethal in the infected individuals.

[Mycobacteria identification in solid culture by inverted phase microscopy and ziehl-neelsen stain].

With the aim of identifying morphological differences in colonies of different species of mycobacteria by means of inverted phase microscopy, nine species of high-prevalence pathogenic mycobacteria were seeded in 7H11 solid medium and were observed for up to 21 days. Additionally, Ziehl-Neelsen (ZN) stains were carried out for each one of them. No morphological variations were identified among the species grown on solid medium; however, it was identified that all are able to form cords in solid medium in an early stage of growth, being corroborated by the Ziehl-Neelsen stain in Mycobacterium tuberculosis.

Host and Toxoplasma gondii genetic and non-genetic factors influencing the development of ocular toxoplasmosis: A systematic review.

Toxoplasmosis is a cosmopolitan infection caused by the apicomplexan parasite Toxoplasma gondii. This infectious disease is widely distributed across the world where cats play an important role in its spread. The symptomatology caused by this parasite is diverse but the ocular affectation emerges as the most important clinical phenotype.

[Incidence and prevalence of candidemia in critically ill patients in Colombia].

Introduction: Candidemia is an infectious complication with high morbidity and mortality in intensive care patients.

Objective: The aim of this study was to determine the incidence and prevalence of candidemia in critically ill patients in Colombian hospitals between 2004 and 2008.

Materials And Methods: Data from microbiologically confirmed candidemia cases, ICU stay and admissions were retrospectively recorded in 7 Colombian hospitals between 2004 and 2008.