Publications by authors named "Jessyka Lima Santos"
Article Synopsis
- The enzyme Homoserine dehydrogenase (PbHSD) from Paracoccidioides brasiliensis was successfully expressed in E. coli, yielding 30 mg per liter of culture with high purity.
- Structural analysis indicates that PbHSD consists of about 45.5% α-helices and 10.5% β-strands, and it functions as a homodimer.
- Kinetic studies show that PbHSD has optimal activity at pH 9.35-9.50, with a Michaelis constant (K) of 224 μM, demonstrating its potential for development as an antifungal drug.
Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi of the genus Paracoccidioides spp., which mainly affects workers in rural regions of Latin America. Although the antifungal agents currently available for the treatment of PCM are effective in controlling the disease, many months are needed for healing, making the side effects and drug interactions relevant.
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