Publications by authors named "Jessie Yunjuan Bao"
To adopt an efficient approach of identifying rare variants possibly related to Hirschsprung disease (HSCR), a pilot study was set up to evaluate the performance of a newly designed protocol for next generation targeted resquencing. In total, 20 Chinese HSCR patients and 20 Chinese sex-matched individuals with no HSCR were included, for which coding sequences (CDS) of 62 genes known to be in signaling pathways relevant to enteric nervous system development were selected for capture and sequencing. Blood DNAs from eight pools of five cases or controls were enriched by PCR-based RainDance technology (RDT) and then sequenced on a 454 FLX platform.
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- The rise of plasmid-mediated carbapenemases like NDM-1 in bacteria poses a serious public health threat due to their resistance to nearly all β-lactam antibiotics and limited treatment options.
- Researchers in Hong Kong identified and sequenced the first NDM-1 producing E. coli isolate, discovering a high-frequency-transferring plasmid (pNDM-HK) that showcased a significant genetic resemblance to plasmids from other bacterial species.
- The study highlights the complex evolution of the NDM-1 gene, its association with mobile genetic elements, and its ability to spread across various bacteria, which complicates infection management.