Publications by authors named "Jessie Vandierendonck"
Toxins (Basel)
August 2023
Article Synopsis
- Molecular cloning and gene expression can be difficult when working with toxic proteins, making it challenging to handle such genes in bacteria.
- Researchers created multi-layer control systems that utilize a combination of transcriptional and translational controls, along with reduced plasmid copy numbers, to clone highly toxic proteins effectively.
- The approach includes using weaker promoters with a riboswitch to control toxin expression, enabling the study of effects in living organisms while allowing for controlled overexpression and purification of less toxic proteins.
