Use of decellularized porcine liver for engineering humanized liver organ.

Background: New bioartificial liver devices are needed to supplement the limited supply of organ donors available for patients with end-stage liver disease. Here, we report the results of a pilot study aimed at developing a humanized porcine liver by transplanting second trimester human fetal hepatocytes (Hfh) co-cultured with fetal stellate cells (Hfsc) into the decellularized matrix of a porcine liver.

Material And Methods: Ischemic livers were removed from 19 Yorkshire swine.