Publications by authors named "Jessica-Jae S Palalay"
Fibroblast Growth Factors and their receptors (FGFRs) comprise a cell signaling module that can stimulate signaling by Ras and the kinases Raf, MEK, and ERK to regulate animal development and homeostatic functions. In Caenorhabditis elegans, the sole FGFR ortholog EGL-15 acts with the GRB2 ortholog SEM-5 to promote chemoattraction and migration by the sex myoblasts (SMs) and fluid homeostasis by the hypodermis (Hyp7). Cell-specific differences in EGL-15 signaling were suggested by the phenotypes caused by egl-15(n1457), an allele that removes a region of its C-terminal domain (CTD) known to bind SEM-5.
View Article and Find Full Text PDFShear force enhances adhesion of by counteracting pilus-driven surface departure.
Proc Natl Acad Sci U S A
October 2023
Fluid flow is thought to prevent bacterial adhesion, but some bacteria use adhesins with catch bond properties to enhance adhesion under high shear forces. However, many studies on bacterial adhesion either neglect the influence of shear force or use shear forces that are not typically found in natural systems. In this study, we use microfluidics and single-cell imaging to examine how the human pathogen interacts with surfaces when exposed to shear forces typically found in the human body (0.
View Article and Find Full Text PDFFluid flow is thought to prevent bacterial adhesion, but some bacteria use adhesins with catch bond properties to enhance adhesion under high shear forces. However, many studies on bacterial adhesion either neglect the influence of shear force or use shear forces that are not typically found in natural systems. In this study, we use microfluidics and single-cell imaging to examine how the human pathogen interacts with surfaces when exposed to shear forces typically found in the human body (0.
View Article and Find Full Text PDFArticle Synopsis
- * Recent interdisciplinary research has shed light on how flow affects various bacterial responses, including adhesion, movement, colonization, and gene expression.
- * The review focuses on four different bacterial species and outlines experimental methods to study their behavior in flow, highlighting that not all responses are due to shear forces and emphasizing flow-sensitive signaling in bacteria.
Article Synopsis
- - Cells in natural environments are affected by fluid flow, but most lab experiments, which use batch cell culture, overlook this factor in studying cell behavior and physiology.
- - Research using microfluidics and single-cell imaging reveals that the combination of fluid flow (shear rate) and chemical stress from hydrogen peroxide affects how human pathogens respond at a genetic level, in ways not seen in standard lab settings.
- - High shear rates in flowing conditions can significantly increase cells' sensitivity to low levels of hydrogen peroxide, aligning experimental conditions more closely with those in the human bloodstream, which helps explain how bacteria adapt to their natural environments.