Respiratory infection- and asthma-prone, low vaccine responder children demonstrate distinct mononuclear cell DNA methylation pathways.

Background: Infants with frequent viral and bacterial respiratory infections exhibit compromised immunity to routine immunizations. They are also more likely to develop chronic respiratory diseases in later childhood. This study investigated the feasibility of epigenetic profiling to reveal endotype-specific molecular pathways with potential for early identification and immuno-modulation.

Neighborhood-level racialized socioeconomic deprivation and contraceptive use in the United States, 2011-2019.

Introduction: The social and structural environments where people live are understudied in contraceptive research. We assessed if neighborhood measures of racialized socioeconomic deprivation are associated with contraceptive use in the United States.

Methods: We used restricted geographic data from four waves of the National Survey of Family Growth (2011-2019) limited to non-pregnant women ages 15-44 who had sex in the last 12 months.

Best for Whom? Heterogeneous Treatment Effects of Breastfeeding on Child Development.

The slogan "Breast is Best" has been popularized by medical organizations and parenting networks to extoll the benefits of breastfeeding, yet the causal effects are widely debated. Our study contributes to the debate by examining whether breastfeeding has differential effects based on the propensity to breastfeed, which is also known as causal effect heterogeneity. Prior studies attempt to isolate the causal effect of breastfeeding by netting out confounding characteristics, but we argue that the effects of breastmilk are unlikely to operate in a vacuum.

Regulation of Brain Primary Cilia Length by MCH Signaling: Evidence from Pharmacological, Genetic, Optogenetic, and Chemogenic Manipulations.

The melanin-concentrating hormone (MCH) system is involved in numerous functions, including energy homeostasis, food intake, sleep, stress, mood, aggression, reward, maternal behavior, social behavior, and cognition. In rodents, MCH acts on MCHR1, a G protein-coupled receptor, which is widely expressed in the brain and abundantly localized to neuronal primary cilia. Cilia act as cells' antennas and play crucial roles in cell signaling to detect and transduce external stimuli to regulate cell differentiation and migration.

The Role of Welfare in Family Income Inequality: 1968-2016.

Income inequality among U.S. families with children has increased over recent decades, coinciding with a period of significant reforms in federal welfare policy.

Robust, flexible, and scalable tests for Hardy-Weinberg equilibrium across diverse ancestries.

Traditional Hardy-Weinberg equilibrium (HWE) tests (the χ2 test and the exact test) have long been used as a metric for evaluating genotype quality, as technical artifacts leading to incorrect genotype calls often can be identified as deviations from HWE. However, in data sets composed of individuals from diverse ancestries, HWE can be violated even without genotyping error, complicating the use of HWE testing to assess genotype data quality. In this manuscript, we present the Robust Unified Test for HWE (RUTH) to test for HWE while accounting for population structure and genotype uncertainty, and to evaluate the impact of population heterogeneity and genotype uncertainty on the standard HWE tests and alternative methods using simulated and real sequence data sets.

Polymerization of Cyclopropenes: Taming the Strain for the Synthesis of Controlled and Sequence-Regulated Polymers.

Cyclopropenes (CPEs) are highly strained cyclic olefins, yet there are surprisingly limited examples leveraging their high strain energy for polymerization. In the past, attempts had been made to polymerize CPEs via cationic and insertion polymerization, but side reactions often gave uncontrolled polymers with mixed backbone structures. Ring-opening metathesis polymerization (ROMP) represents an ideal strategy for polymerizing CPEs to access new types of polymers.

Accuracy of a novel stress echocardiography pattern for myocardial bridging in patients with angina and no obstructive coronary artery disease - A retrospective and prospective cohort study.

Background: Myocardial bridge (MB) may cause angina in patients with no obstructive coronary artery disease (CAD). We previously reported a novel stress echocardiography (SE) pattern of focal septal buckling with apical sparing in the end-systolic to early-diastolic phase that is associated with the presence of an MB. We evaluated the diagnostic accuracy of this pattern, and prospectively validated our results.

Degradable Polyacetals/Ketals from Alternating Ring-Opening Metathesis Polymerization.

We report the synthesis of degradable polyacetals and polyketals with controlled molecular weights and low dispersities using alternating ring-opening metathesis polymerization (AROMP) of 1,1-disubstituted cyclopropenes and dioxepins. Under optimized conditions, high degrees of alternation and controlled polymerization were achieved between nonpropagating cyclopropenes and low-strain dioxepins. The high degrees of alternation allowed the resulting polymers to fully degrade into small molecules under acidic conditions at variable rates depending on the acetal/ketal structures.

Hepatitis B Virus Reactivation in the Setting of Immunosuppressive Drug Therapy.

Chronic hepatitis B virus (HBV) infection remains a global health burden, affecting an estimated 257 million people, and is associated with substantial morbidity and mortality due to cirrhosis and hepatocellular carcinoma. Reactivation of HBV infection among individuals with resolved and/or chronic HBV infection may result in clinical hepatitis with a rise in serum HBV DNA and serum alanine aminotransferase, and delayed identification may result in fulminant hepatitis and fatal liver failure. Routine screening for HBV is recommended in patients undergoing immunosuppressive drug regimens known to be associated with HBV reactivation (HBVr).

Tuning the Reactivity of Cyclopropenes from Living Ring-Opening Metathesis Polymerization (ROMP) to Single-Addition and Alternating ROMP.

Ring-opening metathesis polymerization (ROMP) has become one of the most important living polymerizations. Cyclopropenes (CPEs) remain underexplored for ROMP. Described here is that the simple swap of 1-methyl to 1-phenyl on 1-(benzoyloxymethyl)CPEs elicited strikingly different modes of reactivity, switching from living polymerization to either selective single-addition or living alternating ROMP.

Model-based clustering for identifying disease-associated SNPs in case-control genome-wide association studies.

Genome-wide association studies (GWASs) aim to detect genetic risk factors for complex human diseases by identifying disease-associated single-nucleotide polymorphisms (SNPs). The traditional SNP-wise approach along with multiple testing adjustment is over-conservative and lack of power in many GWASs. In this article, we proposed a model-based clustering method that transforms the challenging high-dimension-small-sample-size problem to low-dimension-large-sample-size problem and borrows information across SNPs by grouping SNPs into three clusters.

New Statistical Methods for Constructing Robust Differential Correlation Networks to characterize the interactions among microRNAs.

The interplay among microRNAs (miRNAs) plays an important role in the developments of complex human diseases. Co-expression networks can characterize the interactions among miRNAs. Differential correlation network is a powerful tool to investigate the differences of co-expression networks between cases and controls.

Synthesis and Mechanochemical Activation of Ladderene-Norbornene Block Copolymers.

We have recently reported a polymechanophore system, polyladderene (PLDE), which dramatically transforms into polyacetylene (PA) upon mechanical stimulation. Herein, we optimized conditions to synthesize unprecedented block copolymers (BCPs) containing a force-responsive block by sequential ring-opening metathesis polymerization of different norbornenes and bromoladderene. Successful extension from PLDE to other blocks required careful timing and low temperatures to preserve the reactivity of the PLDE-appended catalyst.

The best of intentions: Prenatal breastfeeding intentions and infant health.

Health organizations recommend that mothers exclusively breastfeed infants for the first six months of life. The current study contributes to a growing body of research that examines whether the purported benefits of breastfeeding are causal. We systematically evaluated the role of an expectant mother's prenatal breastfeeding intentions, which reflect not only demographic characteristics, but also knowledge, attitudes, and social norms about infant feeding, and therefore serve as a proxy for positive maternal selection into breastfeeding.

A bright organic NIR-II nanofluorophore for three-dimensional imaging into biological tissues.

Fluorescence imaging of biological systems in the second near-infrared (NIR-II, 1000-1700 nm) window has shown promise of high spatial resolution, low background, and deep tissue penetration owing to low autofluorescence and suppressed scattering of long wavelength photons. Here we develop a bright organic nanofluorophore (named p-FE) for high-performance biological imaging in the NIR-II window. The bright NIR-II >1100 nm fluorescence emission from p-FE affords non-invasive in vivo tracking of blood flow in mouse brain vessels.

Unintended Birth and Children's Long-term Mental Health.

Research has examined the proximate effects of unintended birth on infants and young children, but we know relatively little about the longer-term effects. Given that unintended birth is associated with several childhood risk factors, it might set the stage for poor mental health in adulthood. Drawing on rich intergenerational survey data from the National Longitudinal Survey of Youth, 1979 ( N = 3,742), this study used a variety of statistical techniques to examine whether maternal pregnancy intentions are associated with children's depressive symptoms during early adulthood.

Molecular Engineering of the Peptoid Nanosheet Hydrophobic Core.

The relationship between the structure of sequence-defined peptoid polymers and their ability to assemble into well-defined nanostructures is important to the creation of new bioinspired platforms with sophisticated functionality. Here, the hydrophobic N-(2-phenylethyl)glycine (Npe) monomers of the standard nanosheet-forming peptoid sequence were modified in an effort to (1) produce nanosheets from relatively short peptoids, (2) inhibit the aggregation of peptoids in bulk solution, (3) increase nanosheet stability by promoting packing interactions within the hydrophobic core, and (4) produce nanosheets with a nonaromatic hydrophobic core. Fluorescence and optical microscopy of individual nanosheets reveal that certain modifications to the hydrophobic core were well tolerated, whereas others resulted in instability or aggregation or prevented assembly.

Ring-opening metathesis polymerization of 1,2-disubstituted cyclopropenes.

The ring-opening metathesis polymerization (ROMP) of 1,2-disubstituted cyclopropenes (CPs) has been explored for the first time using Grubbs 3rd generation catalyst. A range of 1,2-CPs yielded polymers with controllable MWs and low dispersitities, and allowed the synthesis of block copolymers, absent from secondary metathesis. However, there existed a competing intramolecular termination pathway for these monomers, limiting the timescale for their ROMP to stay living.

Better for Baby? The Retreat From Mid-Pregnancy Marriage and Implications for Parenting and Child Well-being.

Recent decades have seen a significant decline in mid-pregnancy ("shotgun") marriage, particularly among disadvantaged groups, which has contributed to increasing nonmarital birth rates. Despite public and political concern about this shift, the implications for parenting and child well-being are not known. Drawing on a sample of U.

Work Hours, Schedules, and Insufficient Sleep Among Mothers and Their Young Children.

Studies have linked parents' employment, work hours, and work schedules to their own sleep quality and quantity, but it is unclear whether these associations extend to children. The authors used data from the 5-year in-home survey of the Fragile Families and Child Wellbeing Study (N = 1,818) to examine the associations between maternal work hours and schedule and insufficient sleep among disadvantaged mothers and their young children. They found that mothers who worked more than 35 hours per week were more likely to experience insufficient sleep compared to mothers who worked fewer hours, whereas children were more likely to experience insufficient sleep when their mothers worked between 20 and 40 hours.

Measuring Maternal Nonstandard Work in Survey Data.

Surveys differ in the measurement of nonstandard work, such that some surveys require respondents to indicate whether they work either a standard or a nonstandard schedule, whereas others allow respondents to indicate that they work both types of schedules. We test whether these measurement decisions influence the estimated prevalence of maternal nonstandard work, using data from two sources: the Current Population Survey (N = 1,430) and the Fragile Families and Child Wellbeing Study (N = 2,524). Using propensity score techniques, we find that giving respondents the option of reporting work at more than one type of schedule doubles the prevalence of nonstandard work, compared to allowing respondents to indicate only one type of schedule.