The scaffolding protein IQGAP1 enhances EGFR signaling by promoting oligomerization and preventing degradation.

IQGAP1 is a large, multi-domain scaffold that connects and modulates different signaling networks including the one initiated by epidermal growth factor (EGF). In this study, we have used live cell fluorescence imaging methods along with other biochemical techniques to follow the mechanisms used by IQGAP1 to enhance EGF signaling. We show that IQGAP1 enhances EGF signaling by promoting the oligomerization of its receptor, EGFR, upon EGF addition along with concurrent IQGAP oligomerization.

Does the ventricle limit cardiac contraction rate in the anoxic turtle ()? II. and assessment of the prevalence of cardiac arrythmia and atrioventricular block.

Previous studies have reported evidence of atrio-ventricular (AV) block in the oxygen-limited heart. However, if cardiac arrhythmia occurs in live turtles during prolonged anoxia exposure remains unknown. Here, we compare the effects of prolonged anoxic submergence and subsequent reoxygenation on cardiac electrical activity through electrocardiogram (ECG) recordings of 21 °C- and 5 °C-acclimated turtles to assess the prevalence of cardiac arrhythmia.