Factors impacting the regulation of gene expression in .

nitric oxide synthase (saNOS) contributes to oxidative stress resistance, antibiotic tolerance, virulence, and modulation of aerobic and nitrate-based cellular respiration. Despite its involvement in these essential processes, the genetic regulation of expression has not been well characterized. 5' rapid amplification of cDNA ends on RNA isolated from UAMS-1 (USA200 strain) and AH1263 (USA300 strain) revealed that the transcriptional start site mapped to an adenine nucleotide in the predicted Shine-Dalgarno site located 11 bp upstream of the ATG start codon, suggesting that the transcript may have a leaderless organization or may be subject to processing.

Evaluation of Staphylococcus aureus Antibiotic Tolerance Using Kill Curve Assays.

This chapter describes the use of antibiotic kill curves to examine the tolerance of Staphylococcus aureus to any antibiotic of interest. This is done by treating cultures with a super-minimum inhibitory concentration (MIC) of antibiotic and measuring viability over time by colony-forming units (CFUs). Kill curves provide a unique insight into S.

Interplay of Nitric Oxide Synthase (NOS) and SrrAB in Modulation of Metabolism and Virulence.

nitric oxide synthase (saNOS) is a major contributor to virulence, stress resistance, and physiology, yet the specific mechanism(s) by which saNOS intersects with other known regulatory circuits is largely unknown. The SrrAB two-component system, which modulates gene expression in response to the reduced state of respiratory menaquinones, is a positive regulator of expression. Several SrrAB-regulated genes were also previously shown to be induced in an aerobically respiring mutant, suggesting a potential interplay between saNOS and SrrAB.