Publications by authors named "Jessica Fahey"
Article Synopsis
- Promoter hypermethylation and histone deacetylation are key epigenetic changes that silence tumor suppressor genes in cancer, prompting a study on glioma cell lines using inhibitors TSA and 5-AzaC to reactivate these genes.
- Analysis revealed that 653 genes were induced by TSA and 170 by 5-AzaC, with a focus on genes that showed significant reactivation specific to glioma cells versus normal astrocytes.
- Notable findings included the identification of the novel genes BEX1 and BEX2, which were silenced and hypermethylated in tumors, and their reexpression enhanced chemotherapy response, suggesting their potential as tumor suppressors and targets for glioma therapy.
Article Synopsis
- The study presents a complete sequence of human chromosome 15, accompanied by a detailed gene catalogue.
- Chromosome 15 is characterized by high rates of segmental duplication, particularly in two areas which are important for understanding genetic disorders like Prader-Willi and Angelman syndromes.
- The researchers identified that most duplications have a common ancestry and that gaps in the genome sequence may arise from structural differences between genetic variants, contributing to ongoing challenges in mapping the human genome.
Group B Streptococcus (GBS) is an important pathogen of neonates, pregnant women, and immunocompromised individuals. GBS isolates associated with human infection produce one of nine antigenically distinct capsular polysaccharides which are thought to play a key role in virulence. A comparison of GBS polysaccharide structures of all nine known GBS serotypes together with the predicted amino acid sequences of the proteins that direct their synthesis suggests that the evolution of serotype-specific capsular polysaccharides has proceeded through en bloc replacement of individual glycosyltransferase genes with DNA sequences that encode enzymes with new linkage specificities.
View Article and Find Full Text PDFThe National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project.
View Article and Find Full Text PDFThe National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes.
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