Identification, amplification and characterization of miR-17-92 from canine tissue.

Recently, a novel group of genes encoding small RNA molecules, termed microRNAs (miRNAs), has been discovered to play a vital role in eukaryotic gene expression. Known to act in a post-transcriptional fashion, miRNAs can inhibit translation by binding to messenger RNA (mRNA) or by targeting mRNA for degradation. A search of genetic databases revealed significant conservation of miRNA genes between the domestic dog and the human.

Identification of microsatellite markers linked to progressive retinal atrophy in American Eskimo Dogs.

Objective: To identify microsatellite markers linked to progressive retinal atrophy (PRA) in American Eskimo Dogs.

Sample Population: Blood samples or buccal epithelial cells from 66 American Eskimo Dogs, including 53 PRA-unaffected and 13 PRA-affected dogs.

Procedure: The genotypes of unaffected and affected dogs were determined by use of microsatellite markers spanning canine chromosome 9 (CFA09).