In Vivo Optical Clearing of Mammalian Brain.

Established methods for imaging the living mammalian brain have, to date, taken optical properties of the tissue as fixed; we here demonstrate that it is possible to modify the optical properties of the brain itself to significantly enhance at-depth imaging while preserving native physiology. Using a small amount of any of several biocompatible materials to raise the refractive index of solutions superfusing the brain prior to imaging, we could increase several-fold the signals from the deepest cells normally visible and, under both one-photon and two-photon imaging, visualize cells previously too dim to see. The enhancement was observed for both anatomical and functional fluorescent reporters across a broad range of emission wavelengths.

Synthesis of PEG-grafted boron doped Si nanocrystals.

Silicon nanocrystals are intriguing materials for biomedical imaging applications because of their unique optical properties and biological compatibility. We report a new surface functionalization route to synthesize biological buffer soluble and colloidally stable silicon nanocrystals, which is enabled by surface boron doping. Harnessing the distinctive Lewis acidic boron surface sites, postsynthetic modifications of plasma synthesized boron doped nanocrystals were carried out with polyethylene glycol (PEG-OH) ligands in dimethyl sulfoxide under photochemical conditions.