Optimising storage conditions and processing of sheep urine for nitrogen cycle and gaseous emission measurements from urine patches.

In grazing systems, urine patches deposited by livestock are hotspots of nutrient cycling and the most important source of nitrous oxide (NO) emissions. Studies of the effects of urine deposition, including, for example, the determination of country-specific NO emission factors, require natural urine for use in experiments and face challenges obtaining urine of the same composition, but of differing concentrations. Yet, few studies have explored the importance of storage conditions and processing of ruminant urine for use in subsequent gaseous emission experiments.

Meta-analysis of global livestock urine-derived nitrous oxide emissions from agricultural soils.

Nitrous oxide (N O) is an air pollutant of major environmental concern, with agriculture representing 60% of anthropogenic global N O emissions. Much of the N O emissions from livestock production systems result from transformation of N deposited to soil within animal excreta. There exists a substantial body of literature on urine patch N O dynamics, we aimed to identify key controlling factors influencing N O emissions and to aid understanding of knowledge gaps to improve GHG reporting and prioritize future research.

A spatial temporal analysis of the Fusarium graminearum transcriptome during symptomless and symptomatic wheat infection.

Fusarium head blight of wheat is one of the most serious and hazardous crop diseases worldwide. Here, a transcriptomic investigation of Fusarium graminearum reveals a new model for symptomless and symptomatic wheat infection. The predicted metabolic state and secretome of F.

Determination of the factor V Leiden single-nucleotide polymorphism in a commercial clinical laboratory by use of NanoChip microelectronic array technology.

Background: Methods for analysis of the single-nucleotide polymorphism (SNP) known as factor V Leiden (FVL) are described. The technique provides rapid, highly accurate detection of the point mutation that encodes for replacement of arginine-506 with glutamine. After formal assay qualification, 758 clinical samples that had previously been analyzed by the Invader Monoplex Assay were tested as research samples in a commercial clinical laboratory.