Publications by authors named "Jesper Bergmans"
Article Synopsis
- Mitochondrial morphology and membrane potential are key indicators of mitochondrial health, and these can be analyzed using fluorescent dyes in living cells.
- The study focuses on using TMRM and Mitotracker Green FM to assess both mitochondrial shape and membrane potential in primary human skin fibroblasts.
- An integrated protocol is provided for quantifying these parameters through epifluorescence microscopy, specifically highlighting its effectiveness with large, flat cells at high magnification.
Macromolecules of various sizes induce crowding of the cellular environment. This crowding impacts on biochemical reactions by increasing solvent viscosity, decreasing the water-accessible volume and altering protein shape, function, and interactions. Although mitochondria represent highly protein-rich organelles, most of these proteins are somehow immobilized.
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