Background And Aims: Precision-cut tissue slices (PCTS) are widely used as an culture tissue culture technique to study pathogeneses of diseases and drug activities in organs . A novel application of the PCTS model may be in the field of translational research into cholangiopathies such as biliary atresia. Therefore, the aim of this study was to apply the precision-cut slice technique to human bile duct and gallbladder tissue.
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