Methicillin resistance of airborne coagulase-negative staphylococci in homes of persons having contact with a hospital environment.

Background: The persons having contact with a hospital environment (hospital personnel workers and discharged patients) are highly exposed to colonization with multidrug-resistant bacteria. The aim of this study was to evaluate the airborne Staphylococcus genus features in homes in which inhabitants have had contact with the hospital environment.

Methods: Airborne bacteria were collected using a 6-stage Anderson impactor.

[Legal aspects of the supervision of nosocomial infection].

Hospital infections are a serious epidemiological problem since hospital departments have been established. Through many years the problem of hospital infections was unsatisfactorily recognized and not until 1970 appeared in Poland publications concerning hospital infections and suggesting systems of control as well as inspection. In the same time acts and instructions were issued defining principles and procedures in case of nosocomial infections.

[Microbiological and molecular analysis of mycobacterium tuberculosis complex strains isolated from patients with pulmonary tuberculosis].

The aim of the study was to perform microbiological and molecular analysis of Mycobacterium tuberculosis complex strains isolated from patients with pulmonary tuberculosis originating from two different regions of Silesia voivodship in 2000 year. One of 135 strains subjected to the study lacked IS6110 insertion sequence. Its belonging to Mycobacterium tuberculosis complex was confirmed by positive test for specific gyrB gene sequence.

GyrA mutations in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa in a Silesian Hospital in Poland.

Among 73 clinical isolates of Pseudomonas aeruginosa 48 strains were ciprofloxacine (CIP) susceptible and 25 CIP resistant (Minimal inhibitory concentration--MIC>32 microg/ml - 14 strains) or of intermediate susceptibility to CIP (MIC > or = 1,5 - 32 microg/ml - 11 isolates). Mutations in the quinolone-resistance-determining region (QRDR) of gyrA gene were searched in groups of CIP resistant and of intermediate susceptibility to CIP isolates. Two methods: restriction fragment length polymorphism (RFLP) analysis and DNA sequencing analysis allowed to detect three different mutations.

PCR-RFLP analysis of a point mutation in codons 315 and 463 of the katG gene of Mycobacterium tuberculosis isolated from patients in Silesia, Poland.

Resistance to antituberculous agents is an important cause of ineffectiveness of antimicrobial therapy. The resistance of M. tuberculosis to antituberculous agents is a result of mutations in genes participating in those agent's action.

[Fluoroquinolone susceptibility of Pseudomonas aeruginosa strains isolated from clinical materials].

The goal of our research was an analysis of sensitivity of Pseudomonas aeruginosa to fluoroquinolones (SPX, PEF, UB, LOM, CIP, ENX, OFX, NOR). The sensitivity was tested by disk diffusion method, according to NCCLS standards. 120 strains isolated from hospitalized (76 strains) and outpatient clinic (44 strains) persons were tested.