HLA-A gene variation modulates residual function of the pancreatic β-cells in children with type 1 diabetes.

Aim Of The Study: The study aimed to analyze an association between the HLA-A gene variation and a risk of type 1 diabetes development and to evaluate the association of HLA class I and class II alleles with β-cell destruction.

Material And Methods: A group of 108 children with type 1 diabetes were genotyped in HLA-A, -DRB1, and -DQB1 genes using hybridization with oligonu-cleotides probes. Plasma C-peptide concentration was assessed by radioimmunoassay method.

Polymorphism of the thymidylate synthase gene and risk of relapse in childhood ALL.

Polymorphisms of genes encoding proteins involved in drug metabolism can influence the efficacy of leukemia treatment. In this population-wide study we aimed to evaluate selected, metabolically active genetic polymorphisms as prognostic markers of treatment efficacy in acute lymphoblastic leukemia (ALL). A total of 51 cases of leukemia relapse were diagnosed in a group of 354 patients with ALL.

The cross-reactivity of anti-asparaginase antibodies against different L-asparaginase preparations.

Repeated administration of L-asparaginase leads to the development of specific antibodies and hypersensitivity reactions. The aim of the study was to evaluate a possible cross-reaction of anti-asparaginase antibodies, developed against the native E. coli L-asparaginase (Asparaginase Medac), with other preparations of the enzyme.

Additional genetic risk factor for death in children with acute lymphoblastic leukemia: a common polymorphism of the MTHFR gene.

Background: The presence of metabolically important genetic polymorphisms may affect treatment efficacy in patients with malignancies. The objective of this prospective multicenter study was to evaluate the role of selected polymorphisms of genes associated with metabolism of chemotherapeutic drugs as prognostic markers in children with acute lymphoblastic leukemia.

Procedure: Genotyping for the presence of 7 genetic variants in 403 patients and analysis of death cases were performed.

[Metabolic syndrome in young adults type 1 diabetic patients].

Aim: The aim of the study was to estimate the prevalence of metabolic syndrome (MS) in type 1 diabetic patients and to assess the relationship between the scores of MS components and body mass and metabolic control.

Material And Methods: 165 patients aged 18-32 years with diabetes duration 8-26 years were included into the study. The height, weight, waist circumference and blood pressure were measured.

[The rare syndromic forms of monogenic diabetes in childhood].

The most frequent form of diabetes in the childhood is type 1 diabetes. Moreover, the rare forms of diabetes have been also identified in children. Besides of neonatal diabetes caused by the mutations in KCNJ11, SUR1 and GCK genes, other forms of monogenic diabetes are associated with different chronic disorders.

[Idiopathic type 1 diabetes: a heterogenous disease or result of our ignorance?].

Based on etiology a new classification for diabetes mellitus was proposed by the Experts of WHO in 1999. According to the classification, type 1 diabetes was subclassified as type 1A (autoimmune) and type 1B (idiopathic). Both forms result from the destruction of beta cells, however in type 1A the process is immune-mediated but in type 1B the origin of the beta cells impairment is still unknown.

[Tissue-specific autoimmunity in patients with idiopathic diabetes].

Introduction: According to the WHO classification, type 1 diabetes is divided into type 1A - autoimmune and type 1B - idiopathic. The pathogenesis of the latter remains unknown. Clinical observations confirm that type 1 diabetes is often associated with the presence of other organ-specific autoantibodies.

Circadian blood pressure variation in normotensive type 2 diabetes patients and angiotensin converting enzyme polymorphism.

Background/aims: Loss of circadian blood pressure (BP) variation (i.e., lack of nocturnal BP dip by at least 10mmHg, 'non-dipping') is associated with increased mortality rate in subjects with diabetes.

[Acute lymphoblastic leukemia in a child with Sturge-Weber syndrome--case report].

Sturge-Weber syndrome belongs to the group of neuroektomesodermal diseases, so called facomatoses. In contrast to other diseases of this group, there are not any proofs of the genetic determinated background of the syndrome, as well as the higher frequency of cancer. However, the higher frequency of benign astrocytomas was observed.

[Leptin concentration and insulin sensitivity in type 1 diabetic children and adolescents].

The Aim Of The Study: was to estimate the relationship between serum leptin levels and insulin sensitivity and components of metabolic syndrome in type 1 diabetic children and adolescents.

Materials And Methods: 158 patients with type 1 diabetes mellitus (70 girls and 88 boys) aged from 8.2 to 18.

[Insulin glargine improves fasting blood glucose levels in prepubertal children with unsatisfactorily controlled type 1 diabetes].

Unlabelled: The aim of this prospective cross-over study was to compare glycemic control on NPH insulin (NPH) and on glargine in unsatisfactorily controlled type 1 diabetic prepubertal children.

Material And Methods: 14 patients, aged 6-12 years, on multiple insulin injections regimen were included. The study protocol: (i) a 6-month therapy with NPH as basal insulin and (ii) a 6-month therapy with glargine as basal insulin, in a random order.

Prediction of clinical remission using the C-peptide level in type 1 diabetes in children.

Unlabelled: The aim of the study was to identify individual, clinical and metabolic factors that predict the C-peptide levels and appearance of clinical partial remission during the first year of type 1 diabetes (T1D) in children.

Material And Methods: 197 type 1 diabetic patients (84 female and 113 male, mean age 10.4 years) were examined.

Parametric clearance kidney scintigrams; diagnostic potential in diabetes.

Background: The diagnostic usefulness of parametric clearance kidney images was studied in the early diagnosis of diabetic nephropathy, juxtaposed with conventional dynamic urinary investigation (renoscintigraphy) combined with deconvolution procedure of renal and blood time activity curves and determination of plasma clearance of (99m)Tc-ethylenedicysteine ((99m)Tc-EC).

Material And Methods: The investigation was performed on a group of 70 individuals (41 males, 29 females) in whom diabetes type 1 was diagnosed (age 10 to 30 y.; mean 19 y.

[Evaluation of the podocytes in urine in the nephrotic syndrome in childhood].

Unlabelled: The pathogenesis of nephrotic syndrome in childhood is still not clear. In most cases minimal change disease, diffuse mesangial proliferation or focal and segmental glomerulosclerosis (FSGS) cause the nephrotic syndrome. To date many studies have revealed that podocytes are the major players in the pathogenesis.

[Post-exercise microalbuminuria in newly diagnosed type 1 diabetic children - preliminary report].

The Aim: of this study was the evaluation of some factors having an influence on post-exercise albuminuria level in children with newly diagnosed type 1 diabetes as a prognostic factor of developing nephropathy.

Material And Methods: 24 newly diagnosed type 1 diabetic children, aged 5.5-16.

[Helicobacter pylori infection and its metabolic consequences in children and adolescents with type 1 diabetes mellitus].

Unlabelled: Helicobacter pylori infection (Hp) is the cause of chronic gastritis and duodenitis, ulcer disease, and functional gastrointestinal motor disorders. Its prevalence among Polish children with type 1 diabetes mellitus has been estimated at 25%. There is limited data regarding the influence of Helicobacter pylori infection on clinical course of diabetes, with controversial results.

The anti-asparagines antibodies correlate with L-asparagines activity and may affect clinical outcome of childhood acute lymphoblastic leukemia.

The primary aim of the study was to evaluate the importance of anti-asparaginase antibodies for l-asparaginase activity in children with standard and medium risk acute lymphoblastic leukemia (ALL). Forty-seven children with newly diagnosed ALL were included into the prospective study. Enzyme activity and the presence of anti-asparaginase antibodies (IgG and IgM class) were determined.

[Analysis of microvascular density and the expression of vascular-endothelial growth factor (VEGF) and its membrane receptor Flk-1 in neuroblastoma].

Aim: To assess selected angiogenic markers; microvascular density and the expression of VEGF and Flk-1 in relation to clinical features and morphologic types of neuroblastoma.

Patients And Methods: Eighty-two children with neuroblastoma were studied. Morphological assessment was performed in paraffin embedded tissues of the primary tumours.

[Prehypertension in type 1 diabetic children and adolescents].

Background: The elevated blood pressure is one of the most important risk factors of diabetic micro- and macroangiopathy.

Aim Of The Study: Evaluation of the prevalence of prehypertension and relationship between prehypertension, metabolic control and chronic complications in children and adolescents with type 1 diabetes mellitus.

Materials And Methods: 83 patients aged 12.

Electrogastrography in children and adolescents with type 1 diabetes: weak correlation with metabolic control parameters.

Aim: To evaluate gastric myoelectrical activity with respect to duration and metabolic control of type 1 diabetes mellitus (T1DM).

Methods: 172 children and adolescents with T1DM (mean 14.4+/-3.

Influence of interleukin IL-2 and IL-12 + IL-18 on surface expression of immunoglobulin-like receptors KIR2DL1, KIR2DL2, and KIR3DL2 in natural killer cells.

Natural killer (NK) cells express killer cell inhibitory receptors (KIRs) that recognize polymorphic class I MHC molecules. In the present study, we analyze the modulatory effect of IL-2 alone or a combination of IL-12 with IL-18 on surface expression of killer cell immunoglobulin-like receptors KIR2DL1, KIR2DL2, and KIR3DL2 in NK cells. Thus, it was found that IL-2 causes a significant increase in the proportion of cells with given studied receptors.

[Prehypertension risk factors in children and adolescents with type 1 diabetes].

Aim: Higher blood pressure is a marker of increased risk for development of diabetic angiopathy. The aim of the study was to evaluate the selected factors influencing the prevalence of prehypertension in T1DM children and adolescents.

Methodology: 113 T1DM patients (62 male), aged 12-19 years, with diabetes duration from 0.