Gene expression in germinated cysts of Phytophthora nicotianae.

SUMMARY The life cycle of Phytophthora species contains several distinct asexual developmental stages that are important for plant infection and disease development. These include vegetative growth of filamentous hyphae, production of multinucleate sporangia, motile, uninucleate zoospores and germinated cysts, the stage at which plant colonization is initiated. To understand mechanisms regulating molecular and cellular processes in germinated cysts, differential hybridization analysis of a cDNA library was used to identify genes up-regulated after cyst germination in P.

Characterization and evolutionary analysis of a large polygalacturonase gene family in the oomycete plant pathogen Phytophthora cinnamomi.

Polygalacturonases (PGs) are secreted by fungal pathogens during saprophytic and parasitic growth, and their degradation of pectin in the plant cell wall is believed to play a major role in tissue invasion and maceration. In this study, PG activity was demonstrated in culture filtrates of the oomycete plant pathogen, Phytophthora cinnamomi. A P.

The role of proline in osmoregulation in Phytophthora nicotianae.

A cDNA encoding Delta(1)-pyrroline-5-carboxylate reductase (P5CR), the enzyme that catalyzes the final step in proline biosynthesis in plants and bacteria, has been cloned from the oomycete plant pathogen Phytophthora nicotianae. Genomic DNA blots indicated that P. nicotianae and P.