Improving hydrogen and volatile fatty acids production through pretreatment of spent coffee grounds.

Consumption of coffee produces large amounts of waste in the form of spent coffee grounds (SCG), a lignocellulosic material rich in carbohydrates, proteins, and polyphenols. This abundant feedstock is promising in terms of biofuels and value-added product generation. This study investigated the impact of pretreatments, such as alkaline (NaOH), ultrasound, and static magnetic field, on SCG bioconversion in terms of biomolecule release, H potential and volatile fatty acids production.

Biochar confers significant microbial resistance to ammonia toxicity in n-caproic acid production.

Microbial chain elongation integrating innovative bioconversion technologies with organic waste utilization can transition current energy-intensive n-caproic acid production to sustainable circular bioeconomy systems. However, ammonia-rich waste streams, despite their suitability, pose inhibitory challenges to these bioconversion processes. Herein, biochar was employed as an additive to enhance the activity of chain elongating microbes under ammonia inhibition conditions, with an objective to detail underlying mechanisms of improvements.

A perspective on three sustainable hydrogen production technologies with a focus on technology readiness level, cost of production and life cycle environmental impacts.

Hydrogen will play an indispensable role as both an energy vector and as a molecule in essential products in the transition to climate neutrality. However, the optimal sustainable hydrogen production system is not definitive due to challenges in energy conversion efficiency, economic cost, and associated marginal abatement cost. This review summarises and contrasts different sustainable hydrogen production technologies including for their development, potential for improvement, barriers to large-scale industrial application, capital and operating cost, and life-cycle environmental impact.

Design, Construction, and Concept Validation of a Laboratory-Scale Two-phase Reactor to Valorize Whiskey Distillery By-products.

The by-products generated from the whiskey distillation process consist of organic liquids with a high chemical oxygen demand (COD) and residues with a high solid content. Low-carbon strategies that repurpose and valorize such by-products are now imperative to reduce the carbon footprint of the food and beverage industries. The operation of a two-phase anaerobic digester to produce volatile fatty acids (VFAs) and biogas may enable distilleries to transition toward a low-carbon bioeconomy.

Demand-driven biogas production from Upflow Anaerobic Sludge Blanket (UASB) reactors to balance the power grid.

Future energy systems necessitate dispatchable renewable energy to balance electrical grids with high shares of intermittent renewables. Biogas from anaerobic digestion (AD) can generate electricity on-demand. High-rate methanogenic reactors, such as the Upflow Anaerobic Sludge Blanket (UASB), can react quicker to variations in feeding as compared to traditional AD systems.

Two-phase anaerobic digestion for enhanced valorisation of whiskey distillery by-products.

The valorisation of whiskey by-products was assessed and compared in three anaerobic digestion systems. The systems produced similar methane yields, which could satisfy up to 44% of the thermal energy demand at a distillery. Using methane generated from by-products would displace natural gas and reduce the distillery's carbon footprint.

Decarbonising distilled spirits: An assessment of the potential associated with anaerobic digestion of by-products at nine operational distilleries.

This work aims to assess the potential biogas resource of by-products from the production of distilled spirits at 9 operational distilleries in 7 countries. An additional objective was the calculation of the energy resource and Scope 1 greenhouse gas (GHG) emission savings from the use of 21 by-products from the distilleries as a feedstock for anaerobic digestion (AD). To present a holistic perspective on the integration of AD with distilleries, an overview of additional criteria to be considered was provided.

A comparison of digestate management options at a large anaerobic digestion plant.

Increased biogas production from increasing numbers of anaerobic digestion (AD) facilities has increased the mass of digestate applied to agricultural land close to AD plants and has led to an oversupply in some regions. This necessitates long distance digestate transportation accompanied by economic, environmental, and social drawbacks. This work assesses the performance of three different digestate management options (MOs); land application of whole digestate (MO1), digestate separation (MO2), and digestate separation and evaporation (MO3), combined with centralised or decentralised digestate storage.

An assessment of how the properties of pyrochar and process thermodynamics impact pyrochar mediated microbial chain elongation in steering the production of medium-chain fatty acids towards n-caproate.

Microbial chain elongation fermentation is an alternative technology for medium-chain fatty acid (MCFA) production. This paper proposed the addition of pyrochar and graphene in chain elongation to improve MCFA production using ethanol and acetate as substrates. Results showed that the yield of, and selectivity towards, C n-caproate were significantly enhanced with pyrochar addition.

A perspective on the combination of alkali pre-treatment with bioaugmentation to improve biogas production from lignocellulose biomass.

Anaerobic digestion (AD) is a bioprocess technology that integrates into circular economy systems, which produce renewable energy and biofertilizer whilst reducing greenhouse gas emissions. However, improvements in biogas production efficiency are needed in dealing with lignocellulosic biomass. The state-of-the-art of AD technology is discussed, with emphasis on feedstock digestibility and operational difficulty.

Emerging bioelectrochemical technologies for biogas production and upgrading in cascading circular bioenergy systems.

Biomethane is suggested as an advanced biofuel for the hard-to-abate sectors such as heavy transport. However, future systems that optimize the resource and production of biomethane have yet to be definitively defined. This paper assesses the opportunity of integrating anaerobic digestion (AD) with three emerging bioelectrochemical technologies in a circular cascading bioeconomy, including for power-to-gas AD (P2G-AD), microbial electrolysis cell AD (MEC-AD), and AD microbial electrosynthesis (AD-MES).

How can ethanol enhance direct interspecies electron transfer in anaerobic digestion?

Anaerobic digestion (AD) of organic waste to produce biogas is a mature biotechnology commercialised for decades. However, the relatively recent discovery of direct interspecies electron transfer (DIET) brings a new opportunity to improve the efficiency of biogas technology. DIET may replace mediated interspecies electron transfer (MIET) by efficient electron transfer between exoelectrogens and electrotrophic methanogens, thereby enhancing yields and rates of biogas production.

Design, Commissioning, and Performance Assessment of a Lab-Scale Bubble Column Reactor for Photosynthetic Biogas Upgrading with .

The two-step bubble column-photobioreactor photosynthetic biogas upgrading system can enable simultaneous production of biomethane and value-added products from microalgae. However, due to the influence of a large number of variables, including downstream processes and the presence of microalgae, no unanimity has been reached regarding the performance of bubble column reactors in photosynthetic biogas upgrading. To investigate this further, the present work documents in detail, the design and commissioning of a lab-scale bubble column reactor capable of treating up to 16.

Production of Bio-alkanes from Biomass and CO.

Bioelectrochemical technologies such as electro-fermentation and microbial CO electrosynthesis are emerging interdisciplinary technologies that can produce renewable fuels and chemicals (such as carboxylic acids). The benefits of electrically driven bioprocesses include improved production rate, selectivity, and carbon conversion efficiency. However, the accumulation of products can lead to inhibition of biocatalysts, necessitating further effort in separating products.

Effects of foam nickel supplementation on anaerobic digestion: Direct interspecies electron transfer.

Stimulating direct interspecies electron transfer with conductive materials is a promising strategy to overcome the limitation of electron transfer efficiency in syntrophic methanogenesis of industrial wastewater. This paper assessed the impact of conductive foam nickel (FN) supplementation on syntrophic methanogenesis and found that addition of 2.45 g/L FN in anaerobic digestion increased the maximum methane production rate by 27.

A perspective on novel cascading algal biomethane biorefinery systems.

Synergistic opportunities to combine biomethane production via anaerobic digestion whilst cultivating microalgae have been previously suggested in literature. While biomethane is a promising and flexible renewable energy vector, microalgae are increasingly gaining importance as an alternate source of food and/or feed, chemicals and energy for advanced biofuels. However, simultaneously achieving, grid quality biomethane, effective microalgal digestate treatment, high microalgae growth rate, and the most sustainable use of the algal biomass is a major challenge.

Biological hydrogen methanation systems - an overview of design and efficiency.

The rise in intermittent renewable electricity production presents a global requirement for energy storage. Biological hydrogen methanation (BHM) facilitates wind and solar energy through the storage of otherwise curtailed or constrained electricity in the form of the gaseous energy vector biomethane. Biological methanation in the circular economy involves the reaction of hydrogen - produced during electrolysis - with carbon dioxide in biogas to produce methane (4H + CO = CH + 2H), typically increasing the methane output of the biogas system by 70%.

How to optimise photosynthetic biogas upgrading: a perspective on system design and microalgae selection.

Photosynthetic biogas upgrading using microalgae provides a promising alternative to commercial upgrading processes as it allows for carbon capture and re-use, improving the sustainability of the process in a circular economy system. A two-step absorption column-photobioreactor system employing alkaline carbonate solution and flat plate photobioreactors is proposed. Together with process optimisation, the choice of microalgae species is vital to ensure continuous performance with optimal efficiency.

Graphene Facilitates Biomethane Production from Protein-Derived Glycine in Anaerobic Digestion.

Interspecies electron transfer is a fundamental factor determining the efficiency of anaerobic digestion (AD), which involves syntrophy between fermentative bacteria and methanogens. Direct interspecies electron transfer (DIET) induced by conductive materials can optimize this process offering a significant improvement over indirect electron transfer. Herein, conductive graphene was applied in the AD of protein-derived glycine to establish DIET.

Cascading biomethane energy systems for sustainable green gas production in a circular economy.

Biomethane is a flexible energy vector that can be used as a renewable fuel for both the heat and transport sectors. Recent EU legislation encourages the production and use of advanced, third generation biofuels with improved sustainability for future energy systems. The integration of technologies such as anaerobic digestion, gasification, and power to gas, along with advanced feedstocks such as algae will be at the forefront in meeting future sustainability criteria and achieving a green gas supply for the gas grid.

Boosting biomethane yield and production rate with graphene: The potential of direct interspecies electron transfer in anaerobic digestion.

Interspecies electron transfer between bacteria and archaea plays a vital role in enhancing energy efficiency of anaerobic digestion (AD). Conductive carbon materials (i.e.

Study of the performance of a thermophilic biological methanation system.

This study investigated the operation of ex-situ biological methanation at two thermophilic temperatures (55°C and 65°C). Methane composition of 85-88% was obtained and volumetric productivities of 0.45 and 0.

Biogas production generated through continuous digestion of natural and cultivated seaweeds with dairy slurry.

The technical feasibility of long term anaerobic mono-digestion of two brown seaweeds, and co-digestion of both seaweeds with dairy slurry was investigated whilst increasing the organic loading rate (OLR). One seaweed was natural (L. digitata); the second seaweed (S.

Co-generation of biohydrogen and biomethane through two-stage batch co-fermentation of macro- and micro-algal biomass.

Aquatic micro-algae can be used as feedstocks for gaseous biofuel production via biological fermentation. However, micro-algae usually have low C/N ratios, which are not advantageous for fermentation. In this study, carbon-rich macro-algae (Laminaria digitata) mixed with nitrogen-rich micro-algae (Chlorella pyrenoidosa and Nannochloropsis oceanica) were used to maintain a suitable C/N ratio of 20 for a two-stage process combining hydrogen and methane fermentation.

Seasonal variation of chemical composition and biomethane production from the brown seaweed Ascophyllum nodosum.

Ascophyllum nodosum, an abundant Irish brown seaweed, shows significant seasonal variation in chemical composition and biogas production. The polyphenol content is shown to be a more important factor in biogas production than ash content. High polyphenol content in summer months adversely affected biogas production; suggesting two potential harvest dates, March and October.