Swapping the substrate specificities of the neuropeptidases neurolysin and thimet oligopeptidase.

Thimet oligopeptidase (EC 3.4.24.

Crystal structure of human thimet oligopeptidase provides insight into substrate recognition, regulation, and localization.

Thimet oligopeptidase (TOP) is a zinc metallopeptidase that metabolizes a number of bioactive peptides and degrades peptides released by the proteasome, limiting antigenic presentation by MHC class I molecules. We present the crystal structure of human TOP at 2.0-A resolution.