Reversal of Multidrug Resistance in an Epirubicin-Resistant Gastric Cancer Cell Subline.

Background: Gastric cancer is one of the most common malignancies worldwide. Epirubicin (EPI) is used extensively in the treatment of multiple cancers despite its tendency to induce multidrug resistance though overexpression of the ABCB1 efflux pump. However, this overexpression can be disrupted using short interfering RNAs (siRNAs).

Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats.

Purpose: To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC).

Methods: Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I- Control (flap elevation with injection of saline solution) with fifteen animals and II- Experimental (flap elevation with injection of ADSC ) with fifteen animal.

Evaluation of antimicrobial and antitumoral activity of Garcinia mangostana L. (mangosteen) grown in Southeast Brazil.

Purpose: To characterize the anatomy of the fruit and leaf and the presence of phytocompounds. To evaluate the antitumor and antimicrobial activity of ethanolic extract of Garcinia mangostana L. (mangosteen) cultivated in southeastern Brazil.

Development of experimental in vitro burn model.

Purpose: To propose an experimental burn model in NIH-3T3 cell line.

Methods: Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and immunofluorescence.

Results: The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave.

Evaluation of antitumoral and antimicrobial activity of Morinda lcitrifolia L. grown in Southeast Brazil.

Purpose: To evaluate the antitumor and antimicrobial activity of ethanolic extract of Morinda citrifolia L. fruit cultivated in southeastern Brazil.

Methods: Preparation ethanolic extract of the fruit of Morinda citrifolia L.

Adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.

Purpose: To evaluate the effects of the adipose-derived stem cells (ADSC) in the viability of random skin flap in rats.

Methods: Thirty five adult male Wistar rats (weight 250-300 g) were used. ADSC were isolated from adult male rats (n=5).

Characterization of human adipose-derived stem cells.

Purpose: There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs.

Methods: Human adipose precursor cells were obtained from subcutaneous abdominal tissue.

[Morphologic evaluation and Ca2+ mobilization by glicose and acetylcholine in human pancreatic cells].

Aims: The proposal of this study was to analyze morphology of the organelles and cytoskeleton in human pancreatic cells cultured and the mobilization of the cytosolic calcium ([Ca2+]c) in response to glucose and ACh by fluorimetry method.

Material And Methods: The cells were plated on glass coverslips, fixed and stained with a combination of fluorophores: the nuclei were stained with DAPI and mitochondria with Mytotracker Red. It was used phalloidin and the secondary antibodies Alexa Fluor conjugated green and red-fluorescent (488 and 594) to identify the protein cell actin F and type M3 muscarinic receptor respectively.