Publications by authors named "Jerome Estephan"
Article Synopsis
- Researchers have created a new ex vivo model using lung transplant methods to study how SARS-CoV-2 infects human lungs, addressing limitations of animal models and lab systems.
- The study used single-cell RNA sequencing to determine that the virus primarily targets alveolar macrophages (AMs) and monocyte-derived macrophages (MoMacs), with MoMacs showing a stronger inflammatory response.
- Findings indicate that the Wuhan strain of SARS-CoV-2 is more effective than the D614G variant, and understanding how the virus interacts with lung macrophages could inform prevention strategies.
lung perfusion (EVLP) has extended the number of transplantable lungs by reconditioning marginal organs. However, EVLP is performed at 37°C without homeostatic regulation leading to metabolic wastes' accumulation in the perfusate and, as a corrective measure, the costly perfusate is repeatedly replaced during the standard of care procedure. As an interesting alternative, a hemodialyzer could be placed on the EVLP circuit, which was previously shown to rebalance the perfusate composition and to maintain lung function and viability without appearing to impact the global gene expression in the lung.
View Article and Find Full Text PDFIntroduction: Lung transplantation often results in primary and/or chronic dysfunctions that are related to early perioperative innate allo-responses where myeloid subsets play a major role. Corticosteroids are administered upon surgery as a standard-of-care but their action on the different myeloid cell subsets in that context is not known.
Methods: To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours.
In response to the increasing demand for lung transplantation, lung perfusion (EVLP) has extended the number of suitable donor lungs by rehabilitating marginal organs. However despite an expanding use in clinical practice, the responses of the different lung cell types to EVLP are not known. In order to advance our mechanistic understanding and establish a refine tool for improvement of EVLP, we conducted a pioneer study involving single cell RNA-seq on human lungs declined for transplantation.
View Article and Find Full Text PDFArticle Synopsis
- Lung transplantation is the only cure for severe chronic lung diseases but has a 50% survival rate after 5 years, partly due to immune responses.
- Researchers developed a cross-circulatory platform in pigs to study how immune cells are recruited and activated in donor lungs, finding that myeloid cells were the main responders.
- The model allows for easy tracking of immune reactions in real-time, which could help in developing better therapies to improve lung transplant outcomes.
Objective: Tracheobronchial adenoid cystic carcinoma is a rare, slow-growing malignancy with a considerable propensity for local extension that may require complex airway resection to achieve tumor-free margins. The objective of this study was to assess whether our experience supports complex airway resection for tracheobronchial adenoid cystic carcinoma.
Methods: Consecutive patients who underwent curative resection for tracheobronchial adenoid cystic carcinoma at our institution between 1970 and 2019 were included retrospectively and classified as having had complex or standard resection.
Background: Normothermic ex vivo lung perfusion (EVLP) increases the pool of donor lungs by requalifying marginal lungs refused for transplantation through the recovery of macroscopic and functional properties. However, the cell response and metabolism occurring during EVLP generate a nonphysiological accumulation of electrolytes, metabolites, cytokines, and other cellular byproducts which may have deleterious effects both at the organ and cell levels, with impact on transplantation outcomes.
Methods: We analyzed the physiological, metabolic, and genome-wide response of lungs undergoing a 6-h EVLP procedure in a pig model in 4 experimental conditions: without perfusate modification, with partial replacement of fluid, and with adult or pediatric dialysis filters.