Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies.

Replication-defective (RD) recombinant simian virus 40 (SV40)-based gene delivery vectors hold a great potential for clinical applications because of their presumed non-immunogenicity and capacity to induce immune tolerance to the transgene products in humans. However, the clinical use of SV40 vectors has been hampered by the lack of a packaging cell line that produces replication-competent (RC) free SV40 particles in the vector production process. To solve this problem, we have adapted the current SV40 vector genome used for the production of vector particles and generated a novel Vero-based packaging cell line named SuperVero that exclusively expresses the SV40 large T antigen.

Fully Solution-Processed n-i-p-Like Perovskite Solar Cells with Planar Junction: How the Charge Extracting Layer Determines the Open-Circuit Voltage.

Fully solution-processed direct perovskite solar cells with a planar junction are realized by incorporating a cross-linked [6,6]-phenyl-C61-butyric styryl dendron ester layer as an electron extracting layer. Power conversion efficiencies close to 19% and an open-circuit voltage exceeding 1.1 V with negligible hysteresis are delivered.