A liquid medium permitting anaerobic growth of Neisseria gonorrhoeae.

Neisseria gonorrhoeae will grow in an anaerobic atmosphere if provided with nitrite as a terminal electron acceptor, and it is increasingly apparent that this important pathogen may grow anaerobically in vivo. By modifying a previously described chemically-defined medium we have produced a liquid medium capable of supporting growth of N. gonorrhoeae under strictly anaerobic conditions.

A fully defined, clear and protein-free liquid medium permitting dense growth of Neisseria gonorrhoeae from very low inocula.

Neisseria gonorrhoeae is difficult to cultivate in liquid medium. Currently there are no liquid media, defined or undefined, that reliably permit growth of this bacterium from low inocula. Standard clinical laboratory broths may allow multiplication of some strains of gonococci from large inocula, but such media incorporate infusates, extracts or digests and are therefore undefined.