AI-Driven Realtime Monitoring of Early Indicators for Ichthyophthirius multifiliis Infection of Rainbow Trout.

A novel video-based real-time system based on AI (artificial intelligence) was developed to detect clinical signs in fish exposed to pathogens. We selected a White Spot Disease model involving rainbow trout as the experimental animal and the parasitic ciliate Ichthyophthirius multifiliis as a pathogen. We compared two identical fish tank systems: one tank was infected by co-habitation, whereas the other tank was kept non-infected (sham infection).

Time-resolved infrared photoluminescence spectroscopy using parametric three-wave mixing with angle-tuned phase matching.

A setup for time-resolved photoluminescence spectroscopy, based on parametric three-wave mixing in a periodically poled lithium niobate crystal, is characterized. Special attention is given to adjusting the phase matching condition by angle tuning of the luminescent light relative to a strong, continuous-wave laser beam within the crystal. The detection system is capable of operating at room temperature and in a wavelength range from 1.

Infrared hyperspectral upconversion imaging using spatial object translation.

In this paper hyperspectral imaging in the mid-infrared wavelength region is realised using nonlinear frequency upconversion. The infrared light is converted to the near-infrared region for detection with a Si-based CCD camera. The object is translated in a predefined grid by motorized actuators and an image is recorded for each position.

Low-noise mid-IR upconversion detector for improved IR-degenerate four-wave mixing gas sensing.

We compare a nonlinear upconversion detector with a conventional cryogenic InSb detector for the detection of coherent infrared light showing near-shot-noise-limited performance in the upconversion system. The InSb detector is limited by dark noise, which results in a 500 times lower signal-to-noise ratio. The two detectors are compared for the detection of a coherent degenerate four-wave mixing (DFWM) signal in the mid-infrared, and applied to measure trace-level acetylene in a gas flow at atmospheric pressure, probing its fundamental rovibrational transitions.

Infrared upconversion hyperspectral imaging.

In this Letter, hyperspectral imaging in the mid-IR spectral region is demonstrated based on nonlinear frequency upconversion and subsequent imaging using a standard Si-based CCD camera. A series of upconverted images are acquired with different phase match conditions for the nonlinear frequency conversion process. From this, a sequence of monochromatic images in the 3.

Non-collinear upconversion of infrared light.

Two dimensional mid-infrared upconversion imaging provides unique spectral and spatial information showing good potential for mid-infrared spectroscopy and hyperspectral imaging. However, to extract spectral or spatial information from the upconverted images an elaborate model is needed, which includes non-collinear interaction. We derive here a general theory providing the far field of the upconverted light when two arbitrary fields interact inside a nonlinear crystal.

High-resolution mid-IR spectrometer based on frequency upconversion.

We demonstrate a novel approach for high-resolution spectroscopy based on frequency upconversion and postfiltering by means of a scanning Fabry-Perot interferometer. The system is based on sum-frequency mixing, shifting the spectral content from the mid-infrared to the near-visible region allowing for direct detection with a silicon-based CCD camera. This approach allows for low noise detection even without cooling of the detector.

Theory for upconversion of incoherent images.

Upconversion of images is a generic method for shifting the spectral content of entire images. A comprehensive theory for upconversion of incoherent light images is presented and compared against experiments. In particular we consider the important case for upconversion of infinity corrected light.

High-resolution two-dimensional image upconversion of incoherent light.

We consider a technique for high-resolution image upconversion of thermal light. Experimentally, we demonstrate cw upconversion with a resolution of more than 200 × 1000 pixels of thermally illuminated objects. This is the first demonstration (to our knowledge) of high-resolution cw image upconversion.

Effect of long- and short-term exposure to laser light at 1070 nm on growth of Saccharomyces cerevisiae.

The effect of a 1070-nm continuous and pulsed wave ytterbium fiber laser on the growth of Saccharomyces cerevisiae single cells is investigated over a time span of 4 to 5 h. The cells are subjected to optical traps consisting of two counterpropagating plane wave beams with a uniform flux along the x, y axis. Even at the lowest continuous power investigated-i.

Enhanced 2D-image upconversion using solid-state lasers.

Based on enhanced upconversion, we demonstrate a highly efficient method for converting a full image from one part of the electromagnetic spectrum into a new desired wavelength region. By illuminating a metal transmission mask with a 765 nm Gaussian beam to create an image and subsequently focusing the image inside a nonlinear PPKTP crystal located in the high intra-cavity field of a 1342 nm solid-state Nd:YVO(4) laser, an upconverted image at 488 nm is generated. We have experimentally achieved an upconversion efficiency of 40% under CW conditions.

2D optical manipulation and assembly of shape-complementary planar microstructures.

Optical trapping and manipulation offer great flexibility as a non-contact microassembly tool. Its application to the assembly of microscale building blocks may open new doors for micromachine technology. In this work, we demonstrate all-optical assembly of microscopic puzzle pieces in a fluidic environment using programmable arrays of trapping beams.

Fully automated beam-alignment and single stroke guided manual alignment of counter-propagating multi-beam based optical micromanipulation systems.

In a previous paper [J. S. Dam et al, Opt.

Computerized "drag-and-drop" alignment of GPC-based optical micromanipulation system.

In the past, aligning the counterpropagating beams in our 3D real-time generalized phase contrast (GPC) trapping system has been a task requiring moderate skills and prior experience with optical instrumentation. A ray transfer matrix analysis and computer-controlled actuation of mirrors, objective, and sample stage has made this process user friendly. The alignment procedure can now be done in a very short time with just a few drag-and-drop tasks in the user-interface.

Second mode of recycling together with period doubling links single-bubble and multibubble sonoluminescence.

We report the existence of a second type of recycling mode that occurs for air-seeded bubbles. Observation of period doubling in both the stable, the first type, and the second type of recycling mode, together with simultaneous measurement of the relative phase of light emission compared to the drive, shows that the instability boundaries of period doubling and bubble extinction are mainly determined by the bubble size irregardless of the gas composition. The second type mode seems to represent a link between single-bubble and multibubble sonoluminescence.

Size of the light-emitting region in a sonoluminescing bubble.

The size of the light-emitting region is a key parameter toward understanding the light-emitting processes in a sonoluminescing bubble. Here we present measurements of interference effects from particles with a diameter of approximately 2 microm situated 6-10 microm from a sonoluminescing bubble. From the angular size of the pattern and from an estimated distance to the particles we conclude that the light-emitting region of a sonoluminescing bubble is smaller than commonly believed [see, e.

Direct observation of period-doubled nonspherical states in single-bubble sonoluminescence.

We present direct observations of period doubling in the flash to flash pulse heights in single-bubble sonoluminescence. States involved are stable, spherically symmetry broken. Observations are made using seven detectors distributed in the equatorial plane of the bubble.

Stable nonspherical bubble collapse including period doubling in sonoluminescence.

We present observations of stable spherical symmetry broken states in single bubble sonoluminescence including observations of period doubled states. States observed involve both spatially oriented states and states with a tumbling symmetry axis. The observations are made using a fiber based four-channel correlation scheme.

Period-doubling bifurcations from breaking the spherical symmetry in sonoluminescence: experimental verification.

Using a fiber-based four-channel correlation scheme to investigate spatial and temporal correlations, we show that observations of period-doubling phenomena in single bubble sonoluminescence are primarily a result of spontaneously breaking the spherical symmetry in the bubble collapse and, at most, may show up as secondary effects in the flash-to-flash spatially integrated light output.