Glycoproteins of Capsosiphon fulvescens modulate synaptic clustering of PSD95 and prevent social isolation-induced cognitive decline in aged male rats.

Social isolation and loneliness inducing cognitive decline are serious health problems in the elderly. Although the hydrophilic glycoproteins of Capsosiphon fulvescens (Cf-hGP) prevent aging-induced cognitive impairment, its effects on social isolation-induced cognitive dysfunction are unclear. This study investigated the efficacy of Cf-hGP against cognitive dysfunction in aged rats and delineated its underlying mechanisms.

Interaction of JNK and mGluR5 in the regulation of psychomotor behaviours after repeated cocaine administration.

Activation of protein kinases after cocaine administration controls psychomotor behaviours by interacting with metabotropic receptors in the brain. This study identified how c-Jun N-terminal kinase (JNK) interacts with metabotropic glutamate receptor 5 (mGluR5) in vitro and in the caudate and putamen (CPu). The potential role of this interaction in the regulation of psychomotor behaviour was also evaluated after administration of cocaine.

Repeated nicotine exposure increases the intracellular interaction between ERK-mGluR5 in the nucleus accumbens more in adult than adolescent rats.

Intracellular interactions between protein kinases and metabotropic receptors in the striatum regulate behavioral changes in response to drug exposure. We investigated the difference in the degree of interaction between extracellular signal-regulated kinase (ERK) and metabotropic glutamate receptor subtype 5 (mGluR5) in the nucleus accumbens (NAc) after repeated exposure to nicotine in adult and adolescent rats. The results showed that repeated exposure to nicotine (0.

Glycoproteins Enhance Probiotics-Induced Cognitive Improvement in Aged Rats.

Aging-induced cognitive dysfunction can be regulated by probiotics through bidirectional communication with the brain. This study aimed to investigate whether glycoproteins (Cf-hGP) enhanced probiotic-induced improvement of memory in aged rats and the underlying mechanism in the dorsal hippocampus. Cf-hGP were isolated using lectin resin.

Synthesis of Tungsten Carbide Nanomaterials in Triple DC Thermal Plasma Jet System.

The tungsten carbide nanomaterials were synthesized in the triple DC thermal plasma jet system using refractory tungsten, and carbon sources such as multi wall carbon nanotube (MWCNT), amorphous carbon and methane. The starting materials were evaporated in the high temperature region of triple plasma jet, then condensed particles were prepared in nanoscale under 100 nm. The effect of carbon sources was investigated on a view of crystal phase structure and morphology.

Synthesis of Metal Boride Nanoparticles Using Triple Thermal Plasma Jet System.

Titanium, nickel, and tungsten boride nanoparticles were synthesized in the triple thermal plasma jet system. The coalesced high-enthalpy thermal plasma jet not only generates extensive high temperature regions but also allows the starting materials to penetrate into the center of high temperature regions effectively. The synthesis process of metal boride was investigated according to the nucleation temperature of three metals and boron.

Hydrophilic Glycoproteins of an Edible Green Alga Prevent Aging-Induced Spatial Memory Impairment by Suppressing GSK-3β-Mediated ER Stress in Dorsal Hippocampus.

Endoplasmic reticulum (ER) stress is involved in various neurodegenerative disorders. We previously found that () crude proteins enhance spatial memory by increasing the expression of brain-derived neurotrophic factor (BDNF) in rat dorsal hippocampus. The present study investigated whether the chronic oral administration of hydrophilic glycoproteins (Cf-hGP) reduces aging-induced cognitive dysfunction by regulating ER stress in the dorsal hippocampus.

Fucosterol from an Edible Brown Alga Prevents Soluble Amyloid Beta-Induced Cognitive Dysfunction in Aging Rats.

Fucosterol from edible brown seaweeds has various biological activities, including anti-inflammatory, anti-adipogenic, antiphotoaging, anti-acetylcholinesterase, and anti-beta-secretase 1 activities. However, little is known about its effects on soluble amyloid beta peptide (sAβ)-induced endoplasmic reticulum (ER) stress and cognitive impairment. Fucosterol was isolated from the edible brown seaweed , and its neuroprotective effects were analyzed in primary hippocampal neurons and in aging rats.

Activation of Protein Kinase G After Repeated Cocaine Administration Is Necessary for the Phosphorylation of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazolepropionic Acid Receptor GluA1 at Serine 831 in the Rat Nucleus Accumbens.

Phosphorylation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in the striatum plays a crucial role in regulating the receptor-coupled signaling cascades leading to behavioral changes associated with psychostimulant exposure. The present study determined if activation of protein kinase G (PKG) contributes to the phosphorylation of AMPA receptor GluA1 subunit at the position of serine 831 (GluA1-S831) in the rat nucleus accumbens (NAc) after repeated cocaine administration. The results demonstrated that repeated intraperitoneal (i.

Association between preoperative high sensitive troponin I levels and cardiovascular events after hip fracture surgery in the elderly.

Objective: Cardiovascular complications contribute to postoperative morbidity and mortality in elderly hip fracture patients. Limited data are available regarding which preoperative risk factors predict cardiovascular course following hip fracture surgery (HFS). We used high sensitive troponin I (hs-TnI) assays and clinical parameters to identify preoperative risk factors associated with major adverse cardiac events (MACE) in elderly hip fracture patients.

Repeated Administration of Cigarette Smoke Condensate Increases Glutamate Levels and Behavioral Sensitization.

Nicotine, a nicotinic acetylcholine receptor agonist, produces the reinforcing effects of tobacco dependence by potentiating dopaminergic and glutamatergic neurotransmission. Non-nicotine alkaloids in tobacco also contribute to dependence by activating the cholinergic system. However, glutamatergic neurotransmission in the dorsal striatum associated with behavioral changes in response to cigarette smoking has not been investigated.

Phycoerythrin-Derived Tryptic Peptide of a Red Alga Pyropia yezoensis Attenuates Glutamate-Induced ER Stress and Neuronal Senescence in Primary Rat Hippocampal Neurons.

Scope: Glutamate excitotoxicity has been observed in association with neurodegenerative disorders. This study aimed to investigate whether a phycoerythrin-derived tryptic peptide of Pyropia yezoensis (PYP) reduces glutamate-induced excitotoxicity and neuronal senescence in primary rat hippocampal neurons.

Methods And Results: Glutamate exposure (100 μm) decreased cell viability and increased expression of endoplasmic reticulum (ER) stress response protein glucose-regulated protein 78 (GRP78) starting at 60 min following glutamate exposure, which was prevented by pretreating the neurons with PYP (1 μg mL ).

Sewable soft shields for the γ-ray radiation.

Soft shields are required to protect the human body during a radioactive accident. However, the modulus of most soft shields, such as HDPE and epoxy, is high, thereby making it difficult to process them in wearable forms like gloves and clothes. We synthesized a soft shield based on a hydrogel that is very compliant, stretchable, and biocompatible.

Phycoerythrin Peptide from Pyropia yezoensis Alleviates Endoplasmic Reticulum Stress Caused by Perfluorooctane Sulfonate-Induced Calcium Dysregulation.

Perfluorooctane sulfonate (PFOS), a stable fluorosurfactant, causes endoplasmic reticulum (ER) stress in the brain. This study was designed to investigate whether a phycoerythrin-derived peptide of (PYP) reduces PFOS-induced ER stress associated with calcium dysregulation. The protective effects of PYP were determined by cell viability, immunoblotting for ER stress response protein glucose-regulated protein 78 (GRP78) and calcium-dependent protein kinases in rat frontal cortical neurons.

Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress.

Perfluorooctane sulfonate (PFOS) is a stable fluorosurfactant, which causes adverse effects in various organisms. The present study was designed to investigate the effects of Pyropia yezoensis peptide (PYP), a peptide comprised of 11 residues (ALEGGKSSGGG), on PFOS-induced endoplasmic reticulum (ER) stress in Chang cells. PFOS exposure (400 µM) for 24 h significantly decreased cell viability, which was upregulated by 250‑1,000 pg/ml PYP treatment.

Dopamine D4 receptors linked to protein kinase G are required for changes in dopamine release followed by locomotor activity after repeated cocaine administration.

We previously found that the dopamine D2-type receptors (D2 and D3 receptors), coupled to protein kinase G (PKG), upregulate locomotor activity after repeated cocaine administration. In this study, D4 receptors, another type of D2 receptor also coupled to PKG, were examined to determine their requirement in the regulation of locomotor activity after repeated cocaine administration. The results demonstrated that repeated injections of cocaine (20 mg/kg), given once a day for seven consecutive days, significantly increased extracellular dopamine concentrations.

Analysis of the contribution of wind drift factor to oil slick movement under strong tidal condition: Hebei Spirit oil spill case.

The purpose of this study is to investigate the effects of the wind drift factor under strong tidal conditions in the western coastal area of Korea on the movement of oil slicks caused by the Hebei Spirit oil spill accident in 2007. The movement of oil slicks was computed using a simple simulation model based on the empirical formula as a function of surface current, wind speed, and the wind drift factor. For the simulation, the Environmental Fluid Dynamics Code (EFDC) model and Automatic Weather System (AWS) were used to generate tidal and wind fields respectively.

Protein kinase G regulates dopamine release, ΔFosB expression, and locomotor activity after repeated cocaine administration: involvement of dopamine D2 receptors.

Protein kinase G (PKG) activation has been implicated in the regulation of synaptic plasticity in the brain. This study was conducted to determine the involvement of PKG-associated dopamine D2 (D2) receptors in the regulation of dopamine release, ΔFosB expression and locomotor activity in response to repeated cocaine exposure. Repeated systemic injections of cocaine (20 mg/kg), once a day for seven consecutive days, increased cyclic guanosine monophosphate (cGMP) and extracellular dopamine concentrations in the dorsal striatum.

Protein kinase G increases AMPA receptor GluR1 phosphorylation at serine 845 after repeated cocaine administration in the rat nucleus accumbens.

The regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor GluR1 subunit phosphorylation at serine 845 (GluR1-Ser845) by protein kinase G (PKG) activation was investigated in the nucleus accumbens (NAc) after repeated cocaine administration. Intra-NAc injection of the cyclic guanosine monophosphate (cGMP) analog, Rp-8-Br-PET-cGMPS (5 nmol) and the PKG inhibitor, KT5823 (2 nmol), prior to the final drug injection significantly decreased GluR1-Ser845 phosphorylation elevated by repeated systemic injections of cocaine (20mg/kg) once a day for seven consecutive days. The inhibition of PKG also attenuated Ca(2+)-calmodulin-dependent protein kinases II (CaMKII) phosphorylation, however inhibition of CaMKII with KN62 (20 nmol) did not alter the phosphorylation state of GluR1-Ser845.

Protein kinase G linked to dopamine D3 receptors in the dorsal striatum controls dopamine release, ΔFosB expression and locomotor activity after repeated cocaine administration.

Protein kinase G (PKG) has been implicated in a variety of physiological functions including synaptic plasticity in the brain. This study investigated the involvement of dopamine D3 (D3) receptors in PKG-regulated dopamine release, long-term changes in gene expression and behavioral sensitization after repeated cocaine administration. Repeated systemic injections of cocaine (20mg/kg), once a day for seven consecutive days, increased extracellular dopamine concentrations in the dorsal striatum.

Activation of protein kinase C is required for AMPA receptor GluR1 phosphorylation at serine 845 in the dorsal striatum following repeated cocaine administration.

Rationale: Phosphorylation in the α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors plays a crucial role in the regulation of AMPA receptor plasticity associated with drugs of abuse.

Objectives: It is well known that phosphorylation of AMPA receptor GluR1 subunit at serine 845 (S845) is regulated by protein kinase A downstream to dopamine D1 receptors in the striatum. This study was performed to determine whether GluR1-S845 phosphorylation in the rat dorsal striatum is altered by repeated cocaine via a signaling mechanism involving glutamate receptor-associated and Ca(2+)-dependent protein kinases.

Alterations in differentially expressed genes after repeated exposure to perfluorooctanoate and perfluorooctanesulfonate in liver of Oryzias latipes.

Perfluorooctanoate (PFOA) and perfluorooctanesulfonate (PFOS) are considered biologically toxic due to their persistence in the environment. The effects of repeated exposure to these compounds on differentially expressed genes (DEGs) were investigated in liver of the medaka, Oryzias latipes. In this study, seven genes-except for cytochrome P450 3A (CYP450 3A)-were identified as DEGs that were downregulated in response to 15- and 30 days exposures to PFOA and/or PFOS.

Change in labrum height after arthroscopic Bankart repair: correlation with preoperative tissue quality and clinical outcome.

Hypothesis: Arthroscopic factors, such as labral and capsular tissue quality or anterior labral periosteal sleeve avulsion (ALPSA) lesion, affect postoperative labral height stability. Labral height stability has a correlation with clinical outcome.

Methods: The study included 40 patients who underwent arthroscopic surgery for a Bankart lesion between August 2005 and May 2009.

Alterations in differentially expressed genes by exposure to a mixture of carcinogenic polycyclic aromatic hydrocarbons in the liver of Oryzias latipes.

The effects of a mixture of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) on transcriptional responses in the liver of medaka, Oryzias latipes, were investigated by identifying differentially expressed genes (DEGs). Five DEGs were identified as cytochrome P450 2P1 (CYP450 2P1), malate dehydrogenase, anti-thrombin III, NADH dehydrogenase subunit 4, and transferrin. These DEGs were quantified by real-time polymerase chain reaction.