Publications by authors named "Jens Uldum Erlandsen"
Article Synopsis
- Investigated the role of human topoisomerase 1 (TOP1) in regulating G-quadruplex (G4) formation in the Pu27 region of the MYC promoter, where G4 formation inhibits promoter activity.
- Found that downregulating TOP1 increased transcription from a wildtype Pu27 promoter, but this effect was absent if G4 formation was disrupted by mutations; similar effects were observed with another G4 forming element, WT21.
- Demonstrated for the first time that TOP1 can induce G4 formation in double-stranded DNA in vitro and that it selectively interacts with the wildtype Pu27 sequence, highlighting its significant role in G4 dynamics within cells.
Different Camptothecin Sensitivities in Subpopulations of Colon Cancer Cells Correlate with Expression of Different Phospho-Isoforms of Topoisomerase I with Different Activities.
Cancers (Basel)
May 2020
The heterogeneity of tumor cells and the potential existence of rare cells with reduced chemotherapeutic response is expected to play a pivotal role in the development of drug resistant cancers. Herein, we utilized the colon cancer cell lines, Caco2 and DLD1, to investigate heterogeneity of topoisomerase 1 (TOP1) activity in different cell subpopulations, and the consequences for the chemotherapeutic response towards the TOP1 targeting drug, camptothecin. The cell lines consisted of two subpopulations: one (the stem-cell-like cells) divided asymmetrically, was camptothecin resistant, had a differently phosphorylated TOP1 and a lower Casein Kinase II (CKII) activity than the camptothecin sensitive non-stem-cell-like cells.
View Article and Find Full Text PDF