Clinical efficacy of dissolvable microneedles armed with anti-melanogenic compounds to counter hyperpigmentation.

Background: Hyperpigmentation is a complex physiological process associated with alterations of skin color due to melanin overproduction and distribution. Both intrinsic and extrinsic factors influence discoloration with the involvement of multiple pathways in cooperative manners. Restoring natural skin color requires a multi-targeted approach.

Efficacy of bioactive peptides loaded on hyaluronic acid microneedle patches: A monocentric clinical study.

Background: Aging skin is a gradual physiological process associated with functional and structural changes of the skin. Both intrinsic and extrinsic factors influence skin aging by the involvement of multiple pathways. Restoring natural skin conditions requires a multi-targeted approach.

Different effects of CsA and FK506 on aquaporin-2 abundance in rat primary cultured collecting duct cells.

Calcineurin (Cn) inhibitors (CnI) such as cyclosporine A (CsA) and FK506 are nephrotoxic immunosuppressant drugs, which decrease tubular function. Here, we examined the direct effect of CnI on aquaporin-2 (AQP2) expression in rat primary cultured inner medullary collecting duct cells. CsA (0.

Cyclosporin-A induced toxicity in rat renal collecting duct cells: interference with enhanced hypertonicity induced apoptosis.

Background/aims: Rat renal inner medullary collecting duct (IMCD) cells are physiologically exposed to a wide range of ambient tonicity. To maintain their function upon changes in osmolality, IMCD cells induce expression of osmoprotective and antiapoptotic genes, mainly mediated by the transcription factor Tonicity Enhancer Binding Protein (TonEBP). Some drugs like Cyclosporin-A (CsA) are discussed to interfere with the activity of TonEBP and thereby mediate their nephrotoxic effects.

Atrial natriuretic peptide and nitric oxide signaling antagonizes vasopressin-mediated water permeability in inner medullary collecting duct cells.

AVP and atrial natriuretic peptide (ANP) have opposite effects in the kidney. AVP induces antidiuresis by insertion of aquaporin-2 (AQP2) water channels into the plasma membrane of collecting duct principal cells. ANP acts as a diuretic factor.

Translocation of aquaporin-containing vesicles to the plasma membrane is facilitated by actomyosin relaxation.

Docking and fusion of vesicles to the plasma membrane is a fundamental process in living cells. An established model for the trafficking of vesicles is based on primary epithelial cells from the collecting duct of the nephron. Upon stimulation with the signaling peptide arginine-vasopressin (AVP), aquaporin-containing vesicles are directed to the plasma membrane.