Publications by authors named "Jennifer Mitchell Fetch"
Phenolic compounds (PC) in oat may possess health promoting properties. This study evaluated the effect of genotype, environment, and their interaction on the phenolic acid and avenanthramide (AVN) concentration in Canadian oat. Six cultivars were grown at eight locations across Canada in a randomised complete block design with three field replicates.
View Article and Find Full Text PDFArticle Synopsis
- Stem rust is a significant disease affecting cultivated oats, primarily controlled in North America through host resistance from a limited number of resistance genes.
- Researchers focused on a specific resistance gene to develop high-density genetic maps and to create efficient DNA markers for selecting resistant oat varieties.
- Their findings included identifying key markers linked to the resistance gene, which were successfully converted into KASP assays that can accurately predict the presence of the resistance gene in various oat lines and cultivars.
Article Synopsis
- Crown rust, a major disease affecting oats caused by the fungus f. sp. Eriks., leads to significant yield losses and is controlled in part by a gene-for-gene interaction that oat breeders utilize.
- The study aimed to map a specific resistance gene in two independent oat populations and identify SNP markers to predict its presence in breeding material.
- Results showed the resistance gene mapped to linkage group Mrg11 with specific SNP markers, allowing for the development of PCR assays to accurately predict resistance status and improve oat breeding programs through marker-assisted selection.
SNP loci linked to the crown rust resistance gene Pc98 were identified by linkage analysis and KASP assays were developed for marker-assisted selection in breeding programs. Crown rust is among the most damaging diseases of oat and is caused by Puccinia coronata var. avenae f.
View Article and Find Full Text PDFWe adapted and tested a Rapture assay as an enhancement of genotyping-by-sequencing (GBS) in oat (Avena sativa). This assay was based on an additional bait-based capture of specific DNA fragments representing approximately 10,000 loci within the enzyme-based complexity reduction provided by GBS. By increasing the specificity of GBS to include only those fragments that provided effective polymorphic markers, it was possible to achieve deeper sequence coverage of target markers, while simultaneously sequencing a greater number of samples on a single unit of next-generation sequencing.
View Article and Find Full Text PDFArticle Synopsis
- * Pg13 is crucial for stem rust resistance in North American oat cultivars and will aid in routine selection practices like gene pyramiding and backcrosses.
- * High-density linkage maps and genome-wide associations confirmed Pg13's location at approximately 67.7 cM on linkage group Mrg18, coinciding with specific translocation breakpoints and other resistance genes, with reliable KASP assays created for breeding use.
Molecular mapping of crown rust resistance genes is important to effectively utilize these genes and improve breeding efficiency through marker-assisted selection. is a major race-specific crown rust resistance gene initially identified in the wild hexaploid oat in the early 1970s. This gene was transferred to cultivated oat () and has been used as a differential for identification of crown rust races since 1974.
View Article and Find Full Text PDFOat crown rust, caused by f. sp. , is a major constraint to oat ( L.
View Article and Find Full Text PDFArticle Synopsis
- 635 oat lines and 4561 SNP loci were analyzed to understand population structure, linkage disequilibrium (LD), and the relationship between genotypes and heading date.
- The analysis revealed that 25.3% of genetic variation could be explained by the first five principal components, but no clear structured population was found, although clustering indicated differences between spring oats and southern U.S. origins.
- Linkage disequilibrium was observed to decay slower in southern oat lines compared to spring oat lines, particularly highlighting an interesting case on linkage group Mrg28, and several linkage groups were consistently associated with heading date across different environments.
Hexaploid oat ( L., 2 = 6 = 42) is a member of the Poaceae family and has a large genome (∼12.5 Gb) containing 21 chromosome pairs from three ancestral genomes.
View Article and Find Full Text PDFGenetic analysis and genome mapping of a major seedling oat crown rust resistance gene, designated PcKM, are described. The chromosomal location of the PcKM gene was identified and linked markers were validated. Crown rust (Puccinia coronata Corda f.
View Article and Find Full Text PDFArticle Synopsis
- Crown rust, caused by the fungus Puccinia coronata f. sp. avenae, is a major disease affecting oats worldwide, and the oat line MN841801 shows effective adult plant resistance (APR) against it for over 30 years.
- The study examines the genetic basis of this resistance using three recombinant inbred line (RIL) populations and identifies a major quantitative trait locus (QTL) on oat chromosome 14D, called QPc.crc-14D, which accounts for up to 76% of the observed APR.
- This research marks the first identification of a significant APR QTL in oats, establishing QPc.crc-14D as a promising target for marker-assisted breeding and map-based
Article Synopsis
- The study addresses the challenges of creating a consensus map for the complex hexaploid oat genome (Avena sativa), including the size of the genome and scarcity of molecular markers.
- It introduces new methodologies for discovering SNPs and a novel anchoring strategy, successfully resulting in the first complete physically-anchored consensus map that includes 985 SNPs.
- The findings also highlight genetic similarities with other plants, providing tools for detailed genetic analysis and a useful framework for similar research in other complex genomes.
Many core collections have been developed from large collections of crop germplasm, but most of these have not been characterized, particularly using molecular techniques, for germplasm management and utilization. We have attempted to characterize a structured sample representing a world collection of 11,622 cultivated hexaploid oat accessions in the hope of understanding the genetic structure of the world collection. The amplified fragment length polymorphism (AFLP) technique was applied to screen 670 accessions representing 79 countries and one group of uncertain origin.
View Article and Find Full Text PDF