Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates.

The Car9 affinity tag is a dodecameric silica-binding peptide that can be fused to the N- and C-termini of proteins of interest to enable their rapid and inexpensive purification on underivatized silica in a process that typically relies on l-lysine as an eluent. Here, we show that silica paper spin columns and borosilicate multi-well plates used for plasmid DNA purification are suitable for recovering Car9-tagged proteins with high purity in a workflow compatible with high-throughput experiments. Spin columns typically yield 100 μg of biologically active material that can be recovered in minutes with low concentrations of lysine.

Ligand-Modified Human Serum Albumin Nanoparticles for Enhanced Gene Delivery.

The development of nonviral gene delivery systems is a great challenge to enable safe gene therapy. In this study, ligand-modified nanoparticles based on human serum albumin (HSA) were developed and optimized for an efficient gene therapy. Different glutaraldehyde cross-linking degrees were investigated to optimize the HSA nanoparticles for gene delivery.

Autoxidation of platinum(IV) hydrocarbyl hydride complexes to form platinum(IV) hydrocarbyl hydroperoxide complexes.

The platinum(IV) hydride complexes Tp(Me(2))PtR(2)H (Tp(Me(2)) = hydridotris(3,5-dimethylpyrazolyl)borate, R = Me (1a), Ph (1b)) react with molecular oxygen to form platinum(IV) hydroperoxide complexes Tp(Me(2))PtR(2)OOH (R = Me (2a) and Ph (2b), respectively) in high yield. The results of kinetic and mechanistic studies of these reactions are consistent with the net insertion of molecular oxygen into the Pt(IV)-H bonds occurring via radical chain mechanisms. The radical chain pathways resemble, in many respects, those documented for autoxidations of organic substrates, but significant differences are also evident.