Heterologous expression, purification, and characterization of recombinant rat cysteine dioxygenase.

Cysteine dioxygenase (CDO, EC 1.13.11.

Expression, reconstitution, and mutation of recombinant Streptomycescoelicolor NiSOD.

Nickel-dependent superoxide dismutases (NiSODs) represent a novel solution to controlling the deleterious effects of reactive oxygen species derived from superoxide in biology. The expression of recombinant Streptomyces coelicolor NiSOD and its in vitro processing and reconstitution to yield fully active enzyme is reported. The results of studies of NiSODs involving mutations in two putative nickel binding ligands are also reported.