Resident Perceptions of Simulcast Teaching: A Qualitative Study.

Objectives: During the COVID-19 pandemic, medical education programs were challenged to optimize learning while balancing social interaction with exposure risk. In response, our internal medicine (IM) residency program transitioned to "simulcast" educational sessions. In simulcast sessions, multiple small groups of learners met in person in separate rooms and connected to the large-group session via videoconferencing.

Experimentally Induced Pain Results in Reduced Activity of the Rotator Cuff Muscles in Healthy Subjects.

Shoulder pain is a complex, prevalent problem that is multifactorial in nature. While there are many potential causes, one common suspect is the rotator cuff musculature. The purpose of the present study was to induce pain in the supraspinatus muscle of healthy subjects and observe the resulting changes in muscle activity.

USPSTF recommends aspirin to prevent CVD in adults 40 to 59 y as an individual decision; not recommended for adults ≥60 y.

Davidson KW, Barry MJ, Mangione CM, et al. JAMA. 2022;327:1577-84.

Case-Based Insights: Arab Muslim Mothers' Experiences of Managing a Child Newly Diagnosed With Type 1 Diabetes Mellitus.

Mothers frequently fulfill the role of primary caregiver for children diagnosed with type 1 diabetes mellitus (T1DM). A T1DM diagnosis has a significant impact on the child and the wider family unit. The objective is to develop understanding of mothers' experiences caring for children diagnosed with T1DM in the cultural context of the Middle East to facilitate enhanced health service provision and support.

H 1 Proton Channels in Dinoflagellates: Not Just for Bioluminescence?

Bioluminescence in dinoflagellates is controlled by H 1 proton channels. Database searches of dinoflagellate transcriptomes and genomes yielded hits with sequence features diagnostic of all confirmed H 1, and show that H 1 is widely distributed in the dinoflagellate phylogeny including the basal species Oxyrrhis marina. Multiple sequence alignments followed by phylogenetic analysis revealed three major subfamilies of H 1 that do not correlate with presence of theca, autotrophy, geographic location, or bioluminescence.

Spatial factors influence arithmetic performance: the case of the minus sign.

Spatial features of mathematical equations may influence how people solve and interpret those equations. This study examined whether manipulations of spatial features affected how participants solved and interpreted equations involving the minus sign. Undergraduate participants (N = 91) solved multioperation arithmetic equations involving addition, subtraction, and multiplication (e.

TILLING and ecotilling for rice.

Mutagenesis is frequently used to test gene function and to aid in crop improvement. Targeting Induced Local Lesions in Genomes (TILLING) is a reverse genetic strategy first developed to identify induced point mutations in Arabidopsis. This general strategy has since been applied to many plant and animal species.

Fly-TILL: reverse genetics using a living point mutation resource.

Mutagenesis with ethylmethanesulfonate (EMS) has been the standard for traditional genetic screens, and in recent years has been applied to reverse genetics. However, reverse-genetic strategies require maintaining a viable germline library so that mutations that are discovered can subsequently be recovered. In applying our TILLING (Targeting Induced Local Lesions IN Genomes) method to establish a Drosophila reverse-genetic service (Fly-TILL), we chose to screen the Zuker lines, a large collection of EMS-mutagenized second- and third-chromosome balanced lines that had been established for forward-genetic screening.

Retention of induced mutations in a Drosophila reverse-genetic resource.

Targeting induced local lesions in genomes (TILLING) is a reverse-genetic method for identifying point mutations in chemically mutagenized populations. For functional genomics, it is ideal to have a stable collection of heavily mutagenized lines that can be screened over an extended period of time. However, long-term storage is impractical for Drosophila, so mutant strains must be maintained by continual propagation of live cultures.

TILLING to detect induced mutations in soybean.

Background: Soybean (Glycine max L. Merr.) is an important nitrogen-fixing crop that provides much of the world's protein and oil.

Adaptive evolution of the histone fold domain in centromeric histones.

Centromeric DNA, being highly repetitive, has been refractory to molecular analysis. However, centromeric structural proteins are encoded by single-copy genes, and these can be analyzed by using standard phylogenetic tools. The centromere-specific histone, CenH3, replaces histone H3 in centromeric nucleosomes, and is required for the proper distribution of chromosomes during cell division.