Publications by authors named "Jennifer Enders"
Article Synopsis
- The study developed a reverse transcription quantitative polymerase chain reaction (RT-qPCR) method to accurately measure chemically modified small interfering RNA (siRNA), including thermally destabilizing modifications like glycol nucleic acid (GNA).
- RT-qPCR proved to be more sensitive and capable of higher throughput than mass spectrometry for siRNA detection, though mass spectrometry is preferred for specific metabolite detection due to its inefficacy with certain chemical modifications.
- Both RT-qPCR and mass spectrometry have unique advantages and disadvantages, so the choice of method should depend on factors like throughput, sensitivity, and the need for specific metabolite identification.
A novel single-stranded deaminated oligonucleotide metabolite resulting from a REVERSIR™ oligonucleotide was discovered and identified in monkey liver after subcutaneous administration. REVERSIR-A and its metabolites were extracted from biological matrices by solid phase extraction and analyzed using LC coupled with high-resolution MS under negative ionization mode. A novel 9-mer metabolite of REVERSIR-A, resulting from deamination of the 3' terminal 2'--methyl-adenosine nucleotide to 2'--methyl-inosine, was discovered at significant levels in monkey liver.
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