The developmental competence of human metaphase I oocytes with delayed maturation in vitro.

Objective: To evaluate whether metaphase I (MI) oocytes completing maturation in vitro to metaphase II ("MI-MII oocytes") have similar developmental competence as the sibling metaphase II (MII) oocytes that reached maturity in vivo.

Design: Retrospective cohort study.

Setting: Academic medical center.

Metabolomic assessment of oocyte viability.

The aim of the current study was to evaluate whether near-infrared (NIR) spectroscopy-generated metabolomic data obtained from oocyte culture samples would correlate with nuclear maturity status and derived embryo development. A total of 412 oocyte culture samples were collected from 43 patient cycles. Metabolomic profiles of metaphase I and II oocytes were obtained by NIR spectroscopy and were significantly different from each other and from profiles of prophase I (germinal vesicle) oocytes (P +/- 0.

The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer.

Objective: To investigate the role of fast blastocoele re-expansion in the selection of viable thawed blastocysts for transfer.

Design: Retrospective study.

Setting: Academic assisted reproductive program.