Evaluation of Anaplasma spp. seroprevalence in dogs and association with incidence of human anaplasmosis.

Point-of-care (POC) ELISA tests are routinely used in US veterinary practices to screen canine patients for antibodies to tick-transmitted pathogens. Results are also used to monitor spatial and temporal trends in canine seroprevalence, and these data can build awareness of the risk to humans of tick-transmitted diseases such as Lyme disease and anaplasmosis. This study utilized a second-generation test that has incorporated additional Anaplasma-specific peptides into a commercial POC ELISA test to allow detection of Anaplasma spp.

Characterization of canthaxanthin biosynthesis genes from an uncultured marine bacterium.

Carotenoids are isoprenoid pigments synthesized in plants, fungi, bacteria and archaea, with roles in light harvesting, protection from stress, and membrane and protein structures. To characterize carotenoid biosynthesis genes from oceanic microbes, a fosmid library derived from microbial samples collected in surface water of the Pacific Ocean was screened in Escherichia coli for pigment-expressing recombinant strains. One DNA fragment enabled production of a bright orange pigment, and was analysed further by sequence analysis and phenotypic characterization.